Description
Monkeypox Virus (MPXV) D6L Protein IgA ELISA Kit
The Monkeypox Virus D6L Protein Human IgA ELISA Kit is a cutting-edge tool designed for the precise detection of human IgA levels specific to the D6L protein of the monkeypox virus. This advanced kit offers exceptional sensitivity and specificity, guaranteeing accurate and dependable results for a variety of research applications.The D6L protein is a key component of the monkeypox virus, playing a critical role in the virus's pathogenesis and immune response evasion. By measuring the levels of IgA antibodies targeting the D6L protein, researchers can gain valuable insights into the immune response against monkeypox virus infection and potentially develop novel diagnostic and therapeutic strategies.
This Monkeypox Virus D6L Protein Human IgA ELISA Kit is an essential tool for studying monkeypox virus infection, understanding the immune response dynamics, and developing effective countermeasures. Its reliability and precision make it a valuable asset for researchers in virology, immunology, and infectious disease studies.
| Product Name: | Monkeypox Virus (MPXV) D6L Protein IgA ELISA Kit |
| Product Code: | MKRS0020 |
| Species Detected: | Human |
| Gene Symbols: | IGA |
| Compatible Sample Types: | Plasma, Serum |
| Design Principle: | Indirect |
| Number of Targets Detected: | 1 |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Solid Support: | 96-well Microplate |
| Size: | 96T |
- Pre-coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Detection Antibody
- Biotinylated Anti-Positive Control
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- 5X Sample Diluent
Other Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 µl prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
- Add 100 µl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 µl Stop Solution to each well. Read at 450 nm immediately.
