Description
Monkeypox Virus (MPXV) D6L Protein IgG ELISA Kit
The Monkeypox Virus D6L Protein Human IgG ELISA Kit is a cutting-edge tool for accurate detection of the D6L protein specific IgG antibodies in human serum samples. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.The D6L protein is a key component of the Monkeypox virus, playing a crucial role in the viral life cycle and pathogenesis.
Detection of specific IgG antibodies against this protein can aid in understanding immune response to Monkeypox virus infection, as well as in vaccine development and epidemiological studies.Overall, the Monkeypox Virus D6L Protein Human IgG ELISA Kit is an essential tool for researchers and healthcare professionals interested in studying Monkeypox virus and developing strategies for prevention and treatment.
| Product Name: | Monkeypox Virus (MPXV) D6L Protein IgG ELISA Kit |
| Product Code: | MKRS0021 |
| Species Detected: | Human |
| Gene Symbols: | IGG |
| Compatible Sample Types: | Plasma, Serum |
| Design Principle: | Indirect |
| Number of Targets Detected: | 1 |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Solid Support: | 96-well Microplate |
| Size: | 96T |
- Pre-coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Detection Antibody
- Biotinylated Anti-Positive Control
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- 5X Sample Diluent
Other Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 µl prepared Biotinylated detection antibody and Biotinylated Anti-Postive Control into respective wells. Incubate 1 hour at room temperature.
- Add 100 µl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 µl Stop Solution to each well. Read at 450 nm immediately.
