Anti-MRPL13 Antibody (CAB5979)

Antibody Name:	Anti-MRPL13 Antibody
Antibody SKU:	CAB5979
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-179 of human MRPL13 (NP_054797.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SH-SY5Y, HL-60, HT-29, A-549, HeLa, Mouse kidney, Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-179 of human MRPL13 (NP_054797.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSSF SRAP QQWA TFAR IWYL LDGK MQPP GKLA AMAS IRLQ GLHK PVYH ALSD CGDH VVIM NTRH IAFS GNKW EQKV YSSH TGYP GGFR QVTA AQLH LRDP VAIV KLAI YGML PKNL HRRT MMER LHLF PDEY IPED ILKN LVEE LPQP RKIP KRLD EYTQ EEID AFPR LWTP PEDY RL
Gene ID:	28998
Uniprot:	Q9BYD1
Cellular Location:	Mitochondrion
Calculated MW:	20kDa
Observed MW:	20kDa

Synonyms:

MRPL13, L13, L13A, L13mt, RPL13, RPML13

Background:

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein.

UniProt Protein Function:	MRPL13: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:Translation; RNA-binding; Mitochondrial; Ribosomal Chromosomal Location of Human Ortholog: 8q22.1-q22.3 Cellular Component: mitochondrial inner membrane; mitochondrial ribosome; mitochondrial large ribosomal subunit Molecular Function:protein binding; structural constituent of ribosome Biological Process: translation; mitochondrial translation; organelle organization and biogenesis
NCBI Summary:	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9BYD1
NCBI GenInfo Identifier:	22257023
NCBI Gene ID:	28998
NCBI Accession:	Q9BYD1.1
UniProt Secondary Accession:	Q9BYD1,Q9UI04, B2R4R8,
UniProt Related Accession:	Q9BYD1
Molecular Weight:	178
NCBI Full Name:	39S ribosomal protein L13, mitochondrial
NCBI Synonym Full Names:	mitochondrial ribosomal protein L13
NCBI Official Symbol:	MRPL13
NCBI Official Synonym Symbols:	L13; L13A; L13mt; RPL13; RPML13
NCBI Protein Information:	39S ribosomal protein L13, mitochondrial; MRP-L13
UniProt Protein Name:	39S ribosomal protein L13, mitochondrial
Protein Family:	39S ribosomal protein
UniProt Gene Name:	MRPL13
UniProt Entry Name:	RM13_HUMAN

	Western blot analysis of extracts of various cell lines, using MRPL13 antibody (CAB5979) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunohistochemistry of paraffin-embedded rat kidney using MRPL13 Rabbit pAb (CAB5979) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using MRPL13 Rabbit pAb (CAB5979) at dilution of 1:100 (40x lens).

Antibodies

Anti-MRPL13 Antibody (CAB14122)

MRPL13 Antibody (PACO01090)

MRPL13 Antibody (PACO10575)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)