Anti-MRPL28 Antibody (CAB5897)

Antibody Name:	Anti-MRPL28 Antibody
Antibody SKU:	CAB5897
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-256 of human MRPL28 (NP_006419.2).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 IP 1:50 - 1:200
Reactivity:	Human, Rat
Positive Samples:	MCF7, HepG2, 293T, A-549

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-256 of human MRPL28 (NP_006419.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPLH KYPV WLWK RLQL REGI CSRL PGHY LRSL EEER TPTP VHYR PHGA KFKI NPKN GQRE RVED VPIP IYFP PESQ RGLW GGEG WILG QIYA NNDK LSKR LKKV WKPQ LFER EFYS EILD KKFT VTVT MRTL DLID EAYG LDFY ILKT PKED LCSK FGMD LKRG MLLR LARQ DPQL HPED PERR AAIY DKYK EFAI PEEE AEWV GLTL EEAI EKQR LLEE KDPV PLFK IYVA ELIQ QLQQ QALS EPAV VQKR ASGQ
Gene ID:	10573
Uniprot:	Q13084
Cellular Location:	Mitochondrion
Calculated MW:	30kDa
Observed MW:	30kDa

Synonyms:

MRPL28, MAAT1, p15

Background:

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion.

UniProt Protein Function:	MRPL28: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:Ribosomal; Mitochondrial; Translation Chromosomal Location of Human Ortholog: 16p13.3 Cellular Component: nucleoplasm; mitochondrion; cytoplasm; mitochondrial inner membrane; mitochondrial ribosome Molecular Function:protein binding; structural constituent of ribosome Biological Process: translation; mitochondrial translation; organelle organization and biogenesis
NCBI Summary:	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion. [provided by RefSeq, Jul 2008]
UniProt Code:	Q13084
NCBI GenInfo Identifier:	85695426
NCBI Gene ID:	10573
NCBI Accession:	Q13084.4
UniProt Secondary Accession:	Q13084,Q4TT39, Q96S26, Q9BQD8, Q9BR04, B2RCM4, D3DU46
UniProt Related Accession:	Q13084
Molecular Weight:	256
NCBI Full Name:	39S ribosomal protein L28, mitochondrial
NCBI Synonym Full Names:	mitochondrial ribosomal protein L28
NCBI Official Symbol:	MRPL28
NCBI Official Synonym Symbols:	p15; MAAT1
NCBI Protein Information:	39S ribosomal protein L28, mitochondrial; L28mt; MRP-L28; melanoma antigen p15; melanoma-associated antigen recognized by T lymphocytes; melanoma-associated antigen recognized by T-lymphocytes; melanoma-associated antigen recognised by cytotoxic T lymphocytes
UniProt Protein Name:	39S ribosomal protein L28, mitochondrial
UniProt Synonym Protein Names:	Melanoma antigen p15; Melanoma-associated antigen recognized by T-lymphocytes
Protein Family:	39S ribosomal protein
UniProt Gene Name:	MRPL28
UniProt Entry Name:	RM28_HUMAN

	Western blot analysis of extracts of various cell lines, using MRPL28 antibody (CAB5897) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020).
	Immunohistochemistry of paraffin-embedded rat ovary using MRPL28 antibody (CAB5897) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using MRPL28 antibody (CAB5897) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human gastric cancer using MRPL28 antibody (CAB5897) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using MRPL28 antibody (CAB5897). Blue: DAPI for nuclear staining.

Antibodies

MRPL28 Antibody (PACO10583)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)