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MRPL28 Rabbit Monoclonal Antibody (CAB0452)

SKU:
CAB0452
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
MRPL28
MAAT1
p15
mitochondrial ribosomal protein L28
Research Area:
Cell Biology
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:MRPL28 Rabbit Monoclonal Antibody
SKU:CAB0452
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MRPL28 (NP_006419.2).
Sequence:MPLH KYPV WLWK RLQL REGI CSRL PGHY LRSL EEER TPTP VHYR PHGA KFKI NPKN GQRE RVED VPIP IYFP PESQ RGLW GGEG WILG QIYA NNDK LSKR
Tested Applications:WB IP ELISA
Recommended Dilution:WB,1:500 - 1:1000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:p15; MAAT1; MRPL28
Positive Sample:HeLa,293T,K-562,Mouse kidney,Mouse heart,Rat kidney,Rat heart,Ratpancreas
Conjugate:Unconjugated
Cellular Localization:Mitochondrion
Calculated MW:30kDa
Observed MW:30kDa

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion.

Purification Method:Affinity purification
Gene ID:10573
Clone Number:ARC2507
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using MRPL28 Rabbit mAb (CAB0452) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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