Anti-MRPL30 Antibody (CAB16529)

Antibody Name:	Anti-MRPL30 Antibody
Antibody SKU:	CAB16529
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-161 of human MRPL30 (NP_660213.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Rat
Positive Samples:	HeLa, U-251MG, 293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-161 of human MRPL30 (NP_660213.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAGI LRLV VQWP PGRL QTVT KGVE SLIC TDWI RHKF TRSR IPEK VFQA SPED HEKY GGDP QNPH KLHI VTRI KSTR RRPY WEKD IIKM LGLE KAHT PQVH KNIP SVNA KLKV VKHL IRIK PLKL PQGL PAEE NMSN TCLK STGE LVVQ WHLK PVEQ KAHE S
Gene ID:	51263
Uniprot:	Q8TCC3
Cellular Location:	Mitochondrion
Calculated MW:	15kDa/18kDa/21kDa
Observed MW:	18kDa

Synonyms:

MRPL30, L28MT, L30MT, MRP-L28, MRP-L30, MRPL28, MRPL28M, RPML28

Background:

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 6p and 12p. Read-through transcription also exists between this gene and the neighboring upstream lipoyltransferase 1 (LIPT1) gene.

NCBI Summary:	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 6p and 12p. Read-through transcription also exists between this gene and the neighboring upstream lipoyltransferase 1 (LIPT1) gene. [provided by RefSeq, Mar 2011]
UniProt Code:	Q8TCC3
NCBI GenInfo Identifier:	21735604
NCBI Gene ID:	51263
NCBI Accession:	NP_660213.1
UniProt Secondary Accession:	Q8TCC3,Q0D2Q7, Q6ZTP4, Q96Q69, Q9P0N0, A6NIC6, D3DVI0 D3DVI3,
UniProt Related Accession:	Q8TCC3
Molecular Weight:	17
NCBI Full Name:	39S ribosomal protein L30, mitochondrial
NCBI Synonym Full Names:	mitochondrial ribosomal protein L30
NCBI Official Symbol:	MRPL30
NCBI Official Synonym Symbols:	L28MT; L30MT; MRPL28; RPML28; MRP-L28; MRP-L30; MRPL28M
NCBI Protein Information:	39S ribosomal protein L30, mitochondrial
UniProt Protein Name:	39S ribosomal protein L30, mitochondrial
UniProt Synonym Protein Names:	39S ribosomal protein L28, mitochondrial; L28mt; MRP-L28; Mitochondrial large ribosomal subunit protein uL30m
UniProt Gene Name:	MRPL30

	Western blot analysis of extracts of various cell lines, using MRPL30 Rabbit pAb (CAB16529) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded Human breast cancer using MRPL30 Rabbit pAb (CAB16529) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Rat ovary using MRPL30 Rabbit pAb (CAB16529) at dilution of 1:100 (40x lens).

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)