Anti-MRPL45 Antibody (CAB13197)

Antibody Name:	Anti-MRPL45 Antibody
Antibody SKU:	CAB13197
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 57-306 of human MRPL45 (NP_115727.5).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, OVCAR3, 293T, Mouse brain, Mouse heart, Mouse kidney, Mouse liver, Mouse spleen, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 57-306 of human MRPL45 (NP_115727.5).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MQHA RKAG LVIP PEKS DRSI HLAC TAGI FDAY VPPE GDAR ISSL SKEG LIER TERM KKTM ASQV SIRR IKDY DANF KIKD FPEK AKDI FIEA HLCL NNSD HDRL HTLV TEHC FPDM TWDI KYKT VRWS FVES LEPS HVVQ VRCS SMMN QGNV YGQI TVRM HTRQ TLAI YDRF GRLM YGQE DVPK DVLE YVVF EKQL TNPY GSWR MHTK IVPP WAPP KQPI LKTV MIPG PQLK PEEE YEEA QGEA QKPQ LA
Gene ID:	84311
Uniprot:	Q9BRJ2
Cellular Location:	Mitochondrion
Calculated MW:	35kDa
Observed MW:	35kDa

Synonyms:

MRPL45, L45mt, MRP-L45

Background:

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 2p and 17q.

NCBI Summary:	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 2p and 17q. [provided by RefSeq, May 2013]
UniProt Code:	Q9BRJ2
NCBI GenInfo Identifier:	29611869
NCBI Gene ID:	84311
NCBI Accession:	Q9BRJ2.2
UniProt Secondary Accession:	Q9BRJ2,Q6ZMJ5, A1L436,
UniProt Related Accession:	Q9BRJ2
Molecular Weight:	33
NCBI Full Name:	39S ribosomal protein L45, mitochondrial
NCBI Synonym Full Names:	mitochondrial ribosomal protein L45
NCBI Official Symbol:	MRPL45
NCBI Official Synonym Symbols:	L45mt; MRP-L45
NCBI Protein Information:	39S ribosomal protein L45, mitochondrial
UniProt Protein Name:	39S ribosomal protein L45, mitochondrial
UniProt Synonym Protein Names:	Mitochondrial large ribosomal subunit protein mL45
UniProt Gene Name:	MRPL45

	Western blot analysis of extracts of various cell lines, using MRPL45 antibody (CAB13197) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human breast cancer using MRPL45 antibody (CAB13197) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of Hela cells using MRPL45 Rabbit pAb (CAB13197) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using MRPL45 Rabbit pAb (CAB13197) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

MRPL45 Antibody (PACO10590)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)