Anti-MRPS14 Antibody (CAB15498)

Antibody Name:	Anti-MRPS14 Antibody
Antibody SKU:	CAB15498
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-128 of human MRPS14 (NP_071383.1).

Application:	WB IF
Recommended Dilution:	WB 1:200 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	BxPC-3, U-251MG, Mouse heart, Mouse kidney, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-128 of human MRPS14 (NP_071383.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAF MLGS LLRT FKQM VPSS ASGQ VRSH YVDW RMWR DVKR RKMA YEYA DERL RINS LRKN TILP KILQ DVAD EEIA ALPR DSCP VRIR NRCV MTSR PRGV KRRW RLSR IVFR HLAD HGQL SGIQ RATW
Gene ID:	63931
Uniprot:	O60783
Cellular Location:	Mitochondrion
Calculated MW:	15kDa
Observed MW:	15-17kDa

Synonyms:

MRPS14, DJ262D12.2, HSMRPS14, MRP-S14, S14mt

Background:

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S14P family. Alternative splicing results in multiple transcript variants.

NCBI Summary:	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S14P family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010]
UniProt Code:	O60783
NCBI GenInfo Identifier:	11559927
NCBI Gene ID:	63931
NCBI Accession:	NP_071383.1
UniProt Secondary Accession:	O60783,Q5R358,
UniProt Related Accession:	O60783
Molecular Weight:	14
NCBI Full Name:	28S ribosomal protein S14, mitochondrial
NCBI Synonym Full Names:	mitochondrial ribosomal protein S14
NCBI Official Symbol:	MRPS14
NCBI Official Synonym Symbols:	S14mt; MRP-S14; HSMRPS14; DJ262D12.2
NCBI Protein Information:	28S ribosomal protein S14, mitochondrial
UniProt Protein Name:	28S ribosomal protein S14, mitochondrial
UniProt Synonym Protein Names:	Mitochondrial small ribosomal subunit protein uS14m
UniProt Gene Name:	MRPS14

	Western blot analysis of extracts of various cell lines, using MRPS14 antibody (CAB15498) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunofluorescence analysis of HeLa cells using MRPS14 antibody (CAB15498) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using MRPS14 antibody (CAB15498) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-MRPS14 Antibody (CAB15499)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)