The Mouse IGFBP-6 (Insulin-like Growth Factor Binding Protein 6) ELISA Kit - High Sensitivity is a cutting-edge assay meticulously crafted for the precise and sensitive quantification of Insulin-like Growth Factor Binding Protein 6 (IGFBP-6) levels in various biological samples derived from mouse models. IGFBP-6, an essential molecular regulator of the Insulin-like Growth Factor (IGF) system, plays a critical role in modulating cellular responses in growth and development processes. This high-sensitivity ELISA kit provides researchers with an advanced tool to accurately measure IGFBP-6 concentrations, facilitating in-depth investigation of its functions in growth regulation, cell differentiation, and other biological pathways within murine systems. Accurate quantification of IGFBP-6 is fundamental for unraveling its involvement in cellular processes and its potential implications in various physiological and pathological conditions. The Mouse IGFBP-6 (Insulin-like Growth Factor Binding Protein 6) ELISA Kit - High Sensitivity by Assay Genie boasts superior sensitivity and specificity, ensuring dependable and reproducible results. Manufactured under stringent quality control standards, this kit offers exceptional performance and user-friendly operation, making it an excellent choice for cutting-edge research endeavors focusing on the intricate mechanisms governed by IGFBP-6.
Product Name:
Mouse IGFBP-6 ELISA Kit (High Sensitivity)
SKU:
AEES00619
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
75.22 pg/mL
Detection range:
125-8000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
