The Mouse HGF (Hepatocyte Growth Factor) ELISA Kit - High Sensitivity is a state-of-the-art assay meticulously designed for the precise quantitative determination of Hepatocyte Growth Factor (HGF) levels in various biological samples collected from mouse models. HGF, a critical signaling molecule, serves as a key regulator of cellular growth, proliferation, and tissue repair processes, making it a pivotal factor in understanding tissue regeneration, organ development, and pathophysiological conditions in mouse studies. This high-sensitivity ELISA kit empowers researchers to explore the intricate functions of HGF with exceptional sensitivity and accuracy. Through the precise measurement of HGF levels, researchers can unravel the multifaceted roles of this growth factor in cellular dynamics, tissue remodeling, and disease progression in mouse experimental models. The heightened sensitivity of this kit enables detailed investigations into the diverse functions and signaling pathways governed by HGF in mouse biology. The Mouse HGF ELISA Kit - High Sensitivity by Assay Genie guarantees exceptional sensitivity and specificity, ensuring dependable and reproducible results. Crafted under rigorous quality control measures, this kit delivers robust performance and user-friendly operation, making it an ideal solution for researchers aiming to decipher the roles and mechanisms of HGF in mouse-related research endeavors.
Product Name:
Mouse HGF ELISA Kit (High Sensitivity)
SKU:
AEES00602
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
