The Mouse IL-10 ELISA Kit - High Sensitivity is a specialized assay developed for the precise and dependable quantification of Interleukin-10 (IL-10) levels in a variety of biological samples derived from mouse models. IL-10, a crucial anti-inflammatory cytokine, plays a pivotal role in regulating immune responses and maintaining immune system homeostasis. This high-sensitivity ELISA kit empowers researchers to accurately measure IL-10 concentrations, providing valuable insights into its immunomodulatory functions and its impact on inflammation, autoimmune conditions, and infectious diseases within murine systems. Accurate quantification of IL-10 levels is essential for elucidating its role in disease pathogenesis and immune regulatory mechanisms. The Mouse IL-10 ELISA Kit - High Sensitivity by Assay Genie ensures outstanding sensitivity and specificity, guaranteeing credible and reproducible outcomes. Manufactured with meticulous quality control measures, this kit delivers robust performance and user-friendly operation, making it an ideal tool for research endeavors investigating the biological implications of IL-10 in diverse contexts.
Product Name:
Mouse IL-10 ELISA Kit (High Sensitivity)
SKU:
AEES00618
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.13 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
