The Mouse IL-2 ELISA Kit - High Sensitivity is a cutting-edge assay designed for the precise and sensitive quantitation of Interleukin-2 (IL-2) levels in diverse mouse biological samples. IL-2, a vital cytokine, plays a fundamental role in modulating immune responses, particularly in the regulation of T cell proliferation, survival, and differentiation. Its intricate involvement in immune activation, tolerance, and homeostasis highlights its significance in orchestrating cellular immunity and immune-mediated processes. This high-sensitivity ELISA kit enables researchers to delve into the immunological landscape by accurately measuring IL-2 levels, offering key insights into the dynamic immune responses underlying inflammatory reactions, autoimmunity, infectious diseases, and immune-related disorders. Understanding the nuanced functions of IL-2 is essential for deciphering the complexities of immune regulation and developing targeted therapeutic interventions to modulate immune responses effectively. With exceptional sensitivity and specificity, the Mouse IL-2 ELISA Kit - High Sensitivity from Assay Genie ensures dependable and reproducible results, empowering researchers with a robust tool to explore the critical role of IL-2 in immune homeostasis and disease pathogenesis. Manufactured under stringent quality control standards, this kit delivers optimal performance and user-friendly protocols, making it an invaluable asset for in-depth immunological research and drug development endeavors.
Product Name:
Mouse IL-2 ELISA Kit (High Sensitivity)
SKU:
AEES00623
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
