Anti-MSTN Antibody (CAB6913)

Antibody Name:	Anti-MSTN Antibody
Antibody SKU:	CAB6913
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 267-375 of human MSTN (NP_005250.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	NCI-H460, U-937, BT-474, Mouse skeletal muscle, Mouse heart, Mouse liver, Mouse gastrocnemius muscle, Rat skeletal muscle

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 267-375 of human MSTN (NP_005250.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DFGL DCDE HSTE SRCC RYPL TVDF EAFG WDWI IAPK RYKA NYCS GECE FVFL QKYP HTHL VHQA NPRG SAGP CCTP TKMS PINM LYFN GKEQ IIYG KIPA MVVD RCGC S
Gene ID:	2660
Uniprot:	O14793
Cellular Location:	Secreted
Calculated MW:	42kDa
Observed MW:	43kDa

Synonyms:

MSTN, GDF8, MSLHP, myostatin

Background:

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Mutations in this gene are associated with increased skeletal muscle mass in humans and other mammals.

UniProt Protein Function:	MSTN: Acts specifically as a negative regulator of skeletal muscle growth. Defects in MSTN are the cause of muscle hypertrophy (MSLHP). MSLHP is a condition characterized by increased muscle bulk and strength. Affected individuals are exceptionally strong. Belongs to the TGF-beta family.
UniProt Protein Details:	Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 2q32.2 Cellular Component: extracellular space Molecular Function:cytokine activity; identical protein binding; protein binding; receptor binding; transforming growth factor beta receptor binding Biological Process: cell development; muscle development; muscle maintenance; negative regulation of myoblast differentiation; negative regulation of protein kinase B signaling cascade; negative regulation of skeletal muscle growth; positive regulation of transcription, DNA-dependent; regulation of apoptosis; regulation of MAPKKK cascade Disease: Muscle Hypertrophy
UniProt Code:	O14793
NCBI GenInfo Identifier:	4885259
NCBI Gene ID:	2660
NCBI Accession:	NP_005250.1
UniProt Secondary Accession:	O14793,Q6B0H2, A1C2J7, A1C2K0,
UniProt Related Accession:	O14793
Molecular Weight:	42,750 Da
NCBI Full Name:	growth/differentiation factor 8 preproprotein
NCBI Synonym Full Names:	myostatin
NCBI Official Symbol:	MSTN
NCBI Official Synonym Symbols:	GDF8; MSLHP
NCBI Protein Information:	growth/differentiation factor 8
UniProt Protein Name:	Growth/differentiation factor 8
UniProt Synonym Protein Names:	Myostatin
Protein Family:	Growth/differentiation factor
UniProt Gene Name:	MSTN
UniProt Entry Name:	GDF8_HUMAN

	Western blot analysis of extracts of various cell lines, using MSTN antibody (CAB6913) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse lung using MSTN antibody (CAB6913) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
MSTN Antibody (PACO03850)	Human GDF-8 / Myostatin ELISA Kit
MSTN Antibody (PACO10630)	Human MSTN (Myostatin) CLIA Kit (HUES00815)
	Human Growth/differentiation factor 8 (MSTN) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)