Anti-MTFP1 Antibody (CAB7110)

Antibody Name:	Anti-MTFP1 Antibody
Antibody SKU:	CAB7110
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-166 of human MTFP1 (NP_057582.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse liver, Mouse heart, Rat liver, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-166 of human MTFP1 (NP_057582.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSEP QPRG AERD LYRD TWVR YLGY ANEV GEAF RSLV PAAV VWLS YGVA SSYV LADA IDKG KKAG EVPS PEAG RSAR VTVA VVDT FVWQ ALAS VAIP GFTI NRVC AASL YVLG TATR WPLA VRKW TTTA LGLL TIPI IIHP IDRS VDFL LDSS LRKL YPTV GKPS SS
Gene ID:	51537
Uniprot:	Q9UDX5
Cellular Location:	Mitochondrion inner membrane, Multi-pass membrane protein
Calculated MW:	14kDa/18kDa
Observed MW:	25kDa

Synonyms:	MTFP1, HSPC242, MTP18
Background:

UniProt Protein Function:	MTP18: Involved in the mitochondrial division probably by regulating membrane fission. Loss-of-function induces the release of cytochrome c, which activates the caspase cascade and leads to apoptosis. Belongs to the MTFP1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Mitochondrial; Membrane protein, multi-pass; Membrane protein, integral Chromosomal Location of Human Ortholog: 22q Cellular Component: mitochondrial inner membrane; integral to membrane Biological Process: mitochondrial fission; apoptosis
NCBI Summary:	MTP18 is a mitochondrial protein and downstream target of the phosphatidylinositol 3-kinase (see PIK3CA, MIM 171834) signaling pathway that plays a role in cell viability and mitochondrial dynamics (Tondera et al., 2004 [PubMed 15155745]).[supplied by OMIM, Mar 2008]
UniProt Code:	Q9UDX5
NCBI GenInfo Identifier:	52783151
NCBI Gene ID:	51537
NCBI Accession:	Q9UDX5.1
UniProt Secondary Accession:	Q9UDX5,Q9H3K1, Q9P0N6, A6NFQ5,
UniProt Related Accession:	Q9UDX5
Molecular Weight:	166
NCBI Full Name:	Mitochondrial fission process protein 1
NCBI Synonym Full Names:	mitochondrial fission process 1
NCBI Official Symbol:	MTFP1
NCBI Official Synonym Symbols:	MTP18; HSPC242
NCBI Protein Information:	mitochondrial fission process protein 1; mitochondrial 18 kDa protein; mitochondrial protein 18 kDa; mitochondrial fission protein MTP18
UniProt Protein Name:	Mitochondrial fission process protein 1
UniProt Synonym Protein Names:	Mitochondrial 18 kDa protein; MTP18
Protein Family:	Mitochondrial fission process protein
UniProt Gene Name:	MTFP1
UniProt Entry Name:	MTFP1_HUMAN

	Western blot analysis of extracts of various cell lines, using MTFP1 antibody (CAB7110) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human colon carcinoma using MTFP1 antibody (CAB7110) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human breast cancer using MTFP1 antibody (CAB7110) at dilution of 1:100 (40x lens).

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)