The MYBPC3 Antibody (CAB12351) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced through rabbit immunization, is highly specific to human samples and has been validated for use in Western blot applications. By targeting the MYBPC3 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an essential tool for studies in cardiology and genetic heart disorders.MYBPC3, or myosin binding protein C3, plays a significant role in regulating cardiac muscle function and contraction. Mutations in the MYBPC3 gene are known to cause various forms of familial hypertrophic cardiomyopathy, a condition characterized by an enlarged and thickened heart muscle.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
MYBPC3 Antibody
SKU:
CAB12351
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
A Band, C Zone, Cardiac Myofibril, Cytosol, Sarcomere, Striated Muscle Myosin Thick Filament.
Calculated MW:
141kDa
Observed MW:
170kDa
MYBPC3 encodes the cardiac isoform of myosin-binding protein C. Myosin-binding protein C is a myosin-associated protein found in the cross-bridge-bearing zone (C region) of A bands in striated muscle. MYBPC3 is expressed exclusively in heart muscle and is a key regulator of cardiac contraction. Mutations in this gene are a frequent cause of familial hypertrophic cardiomyopathy.
Purification Method
Affinity purification
Gene ID
4607
RRID
AB_2759194
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from rat heart, using MYBPC3 Rabbit pAb (CAB12351) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Rat heart using MYBPC3 Rabbit pAb (CAB12351) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
