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Anti-MYCN Antibody (CAB0499)

SKU:
CAB0499
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
  • Epigenetics and Nuclear Signaling Antibodies 1 Anti-MYCN Antibody CAB0499
  • Epigenetics and Nuclear Signaling Antibodies 1 Anti-MYCN Antibody CAB0499
  • Epigenetics and Nuclear Signaling Antibodies 1 Anti-MYCN Antibody CAB0499
€139 - €419
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Description

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Antibody Name:Anti-MYCN Antibody
Antibody SKU:CAB0499
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 168-267 of human MYCN (NP_005369.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:SH-SY5Y, Mouse heart, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 168-267 of human MYCN (NP_005369.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:AGRA GAAL PAEL AHPA AECV DPAV VFPF PVNK REPA PVPA APAS APAA GPAV ASGA GIAA PAGA PGVA PPRP GGRQ TSGG DHKA LSTS GEDT LSDS DDED
Gene ID:4613
Uniprot:P04198
Cellular Location:Nucleus
Calculated MW:49kDa
Observed MW:49kDa
Synonyms:MODED, N-myc, NMYC, ODED, bHLHe37, MYCN
Background:This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:N-Myc: a proto-oncogenic transcription factor. Plays a role in cell proliferation, apoptosis and in the development of human tumors.
UniProt Protein Details:

Protein type:Oncoprotein; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 2p24.3

Cellular Component: nucleus; chromatin

Molecular Function:protein dimerization activity; protein binding; DNA binding; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; branching morphogenesis of a tube; positive regulation of mesenchymal cell proliferation; negative regulation of astrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; lung development; cartilage condensation; embryonic skeletal morphogenesis

Disease: Feingold Syndrome 1; Tracheoesophageal Fistula With Or Without Esophageal Atresia

NCBI Summary:This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]
UniProt Code:P04198
NCBI GenInfo Identifier:127604
NCBI Gene ID:4613
NCBI Accession:P04198.2
UniProt Secondary Accession:P04198,Q53XS5, Q6LDT9,
UniProt Related Accession:P04198
Molecular Weight:65kDa
NCBI Full Name:N-myc proto-oncogene protein
NCBI Synonym Full Names:v-myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog
NCBI Official Symbol:MYCN  
NCBI Official Synonym Symbols:NMYC; ODED; MODED; N-myc; bHLHe37  
NCBI Protein Information:N-myc proto-oncogene protein; pp65/67; oncogene NMYC; neuroblastoma MYC oncogene; class E basic helix-loop-helix protein 37; neuroblastoma-derived v-myc avian myelocytomatosis viral related oncogene
UniProt Protein Name:N-myc proto-oncogene protein
UniProt Synonym Protein Names:Class E basic helix-loop-helix protein 37; bHLHe37
Protein Family:N-myc protein
UniProt Gene Name:MYCN  
UniProt Entry Name:MYCN_HUMAN
Anti-MYCN Antibody (CAB0499)Western blot analysis of extracts of various cell lines, using MYCN antibody (CAB0499) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
Anti-MYCN Antibody (CAB0499)Immunohistochemistry of paraffin-embedded mouse brain using MYCN antibody (CAB0499) (40x lens).
Anti-MYCN Antibody (CAB0499)Immunohistochemistry of paraffin-embedded rat brain using MYCN antibody (CAB0499) (40x lens).

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