Anti-MYO1C Antibody (CAB6936)

Antibody Name:	Anti-MYO1C Antibody
Antibody SKU:	CAB6936
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 769-1028 of human MYO1C (NP_203693.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HT-29, SKOV3, Mouse lung, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 769-1028 of human MYO1C (NP_203693.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ENAF FLDH VRTS FLLN LRRQ LPQN VLDT SWPT PPPA LREA SELL RELC IKNM VWKY CRSI SPEW KQQL QQKA VASE IFKG KKDN YPQS VPRL FIST RLGT DEIS PRVL QALG SEPI QYAV PVVK YDRK GYKP RSRQ LLLT PNAV VIVE DAKV KQRI DYAN LTGI SVSS LSDS LFVL HVQR ADNK QKGD VVLQ SDHV IETL TKTA LSAN RVNS ININ QGSI TFAG GPGR DGTI DFTP GSEL LITK AKNG HLAV VAPR LNSR
Gene ID:	4641
Uniprot:	O00159
Cellular Location:	Cell membrane, Cell projection, Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle, Nucleus, Nucleus, Peripheral membrane protein, nuclear pore complex, nucleolus, nucleoplasm, ruffle, stereocilium membrane
Calculated MW:	117kDa/119kDa/121kDa
Observed MW:	122kDa

Synonyms:

MYO1C, MMI-beta, MMIb, NMI, myr2, myosin IC

Background:

This gene encodes a member of the unconventional myosin protein family, which are actin-based molecular motors. The protein is found in the cytoplasm, and one isoform with a unique N-terminus is also found in the nucleus. The nuclear isoform associates with RNA polymerase I and II and functions in transcription initiation. The mouse ortholog of this protein also functions in intracellular vesicle transport to the plasma membrane. Multiple transcript variants encoding different isoforms have been found for this gene. The related gene myosin IE has been referred to as myosin IC in the literature, but it is a distinct locus on chromosome 19.

UniProt Protein Function:	MYO1C: Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. Involved in glucose transporter recycling in response to insulin by regulating movement of intracellular GLUT4-containing vesicles to the plasma membrane. Component of the hair cell's (the sensory cells of the inner ear) adaptation-motor complex. Acts as a mediator of adaptation of mechanoelectrical transduction in stereocilia of vestibular hair cells. Binds phosphoinositides and links the actin cytoskeleton to cellular membranes. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Actin-binding; Contractile; Motor; Motility/polarity/chemotaxis; Nucleolus; Lipid-binding Chromosomal Location of Human Ortholog: 17p13.3 Cellular Component: myosin I complex; microvillus; mitochondrion; cytoplasmic membrane-bound vesicle; nuclear pore; cytosol; lipid raft; nucleoplasm; ruffle; filamentous actin; membrane; cytoplasm; unconventional myosin complex; stress fiber; nucleolus; basal plasma membrane; plasma membrane; brush border; lateral plasma membrane Molecular Function:calmodulin binding; protein C-terminus binding; protein binding; Ral GTPase binding; motor activity; actin-dependent ATPase activity; actin binding; ATP binding; receptor binding Biological Process: mRNA transport; metabolic process; innate immune response; protein targeting to membrane; protein targeting; positive regulation of cell migration
NCBI Summary:	This gene encodes a member of the unconventional myosin protein family, which are actin-based molecular motors. The protein is found in the cytoplasm, and one isoform with a unique N-terminus is also found in the nucleus. The nuclear isoform associates with RNA polymerase I and II and functions in transcription initiation. The mouse ortholog of this protein also functions in intracellular vesicle transport to the plasma membrane. Multiple transcript variants encoding different isoforms have been found for this gene. The related gene myosin IE has been referred to as myosin IC in the literature, but it is a distinct locus on chromosome 19. [provided by RefSeq, Jul 2008]
UniProt Code:	O00159
NCBI GenInfo Identifier:	317373577
NCBI Gene ID:	4641
NCBI Accession:	O00159.4
UniProt Related Accession:	O00159
Molecular Weight:	~ 122kDa
NCBI Full Name:	Unconventional myosin-Ic
NCBI Synonym Full Names:	myosin IC
NCBI Official Symbol:	MYO1C
NCBI Official Synonym Symbols:	NMI; MMIb; myr2; MMI-beta
NCBI Protein Information:	unconventional myosin-Ic
UniProt Protein Name:	Unconventional myosin-Ic
UniProt Synonym Protein Names:	Myosin I beta; MMI-beta; MMIb
Protein Family:	Unconventional myosin
UniProt Gene Name:	MYO1C
UniProt Entry Name:	MYO1C_HUMAN

	Western blot analysis of extracts of various cell lines, using MYO1C antibody (CAB6936) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat kidney using MYO1C antibody (CAB6936) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using MYO1C antibody (CAB6936) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of MCF7 cells using MYO1C antibody (CAB6936).

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)