The MYOT Antibody (CAB6439) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific to human myotilin and is validated for use in Western blot applications. By binding to the myotilin protein, this antibody allows for detection and analysis in a variety of cell types, making it ideal for research in muscle biology and related fields.Myotilin is a key player in muscle organization and function, making it a crucial target for studies on muscle diseases and disorders. Dysregulation of myotilin has been linked to muscle-wasting conditions such as myofibrillar myopathies, highlighting the importance of understanding its role in muscle health.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MYOT Antibody
SKU:
CAB6439
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MFM3, TTID, TTOD, LGMD1, LGMD1A, MYOT
Positive Sample:
293T, HepG2, Mouse skeletal muscle, Mouse heart, Rat skeletal muscle, Rat heart
Cellular Localization:
Cell Membrane, Cytoplasm, Z Line, Cytoskeleton, Myofibril, Sarcolemma, Sarcomere.
Calculated MW:
55kDa
Observed MW:
55kDa
This gene encodes a cystoskeletal protein which plays a significant role in the stability of thin filaments during muscle contraction. This protein binds F-actin, crosslinks actin filaments, and prevents latrunculin A-induced filament disassembly. Mutations in this gene have been associated with limb-girdle muscular dystrophy and myofibrillar myopathies. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
Purification Method
Affinity purification
Gene ID
9499
RRID
AB_2767041
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MYOT Rabbit pAb (CAB6439) at 1:1000 dilution._Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using MYOT Rabbit pAb (CAB6439). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
