Anti-NAA10 Antibody (CAB7009)

Antibody Name:	Anti-NAA10 Antibody
Antibody SKU:	CAB7009
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 161-235 of human NAA10 (NP_003482.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, HeLa, HL-60, HepG2, HT-29, Mouse thymus, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 161-235 of human NAA10 (NP_003482.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	HLEL KEKG RHVV LGAI ENKV ESKG NSPP SSGE ACRE EKGL AAED SGGD SKDL SEVS ETTE STDV KDSS EASD SAS
Gene ID:	8260
Uniprot:	P41227
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	24kDa/26kDa
Observed MW:	26kDa

Synonyms:

NAA10, ARD1, ARD1A, ARD1P, DXS707, MCOPS1, NATD, OGDNS, TE2

Background:

N-alpha-acetylation is among the most common post-translational protein modifications in eukaryotic cells. This process involves the transfer of an acetyl group from acetyl-coenzyme A to the alpha-amino group on a nascent polypeptide and is essential for normal cell function. This gene encodes an N-terminal acetyltransferase that functions as the catalytic subunit of the major amino-terminal acetyltransferase A complex. Mutations in this gene are the cause of Ogden syndrome. Alternate splicing results in multiple transcript variants.

UniProt Protein Function:	NAA10: In complex with NAA15, displays alpha (N-terminal) acetyltransferase activity. Without NAA15, displays epsilon (internal) acetyltransferase activity towards HIF1A, thereby promoting its degradation. Represses MYLK kinase activity by acetylation, and thus represses tumor cell migration. Interacts with HIF1A (via its ODD domain); the interaction increases HIF1A protein stability during normoxia, and down-regulates it when induced by hypoxia. Interacts with NAA15, NAA50 and with the ribosome. Binds to MYLK. Ubiquitous. Belongs to the acetyltransferase family. ARD1 subfamily.
UniProt Protein Details:	Protein type:Amino Acid Metabolism - tyrosine; Secondary Metabolites Metabolism - limonene and pinene degradation; Amino Acid Metabolism - phenylalanine; EC 2.3.1.88; Lipid Metabolism - glycerophospholipid; Acetyltransferase Chromosomal Location of Human Ortholog: Xq28 Cellular Component: membrane; cytoplasm; nucleolus; intracellular; nucleus Molecular Function:peptide alpha-N-acetyltransferase activity; N-acetyltransferase activity; protein binding; acetyltransferase activity; ribosome binding Biological Process: N-terminal protein amino acid acetylation; N-terminal peptidyl-glutamic acid acetylation; internal protein amino acid acetylation; N-terminal peptidyl-serine acetylation; DNA packaging Disease: Ogden Syndrome; Microphthalmia, Syndromic 1
NCBI Summary:	N-alpha-acetylation is among the most common post-translational protein modifications in eukaryotic cells. This process involves the transfer of an acetyl group from acetyl-coenzyme A to the alpha-amino group on a nascent polypeptide and is essential for normal cell function. This gene encodes an N-terminal acetyltransferase that functions as the catalytic subunit of the major amino-terminal acetyltransferase A complex. Mutations in this gene are the cause of Ogden syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]
UniProt Code:	P41227
NCBI GenInfo Identifier:	728880
NCBI Gene ID:	8260
NCBI Accession:	P41227.1
UniProt Secondary Accession:	P41227,A6NM98,
UniProt Related Accession:	P41227
Molecular Weight:	235
NCBI Full Name:	N-alpha-acetyltransferase 10
NCBI Synonym Full Names:	N(alpha)-acetyltransferase 10, NatA catalytic subunit
NCBI Official Symbol:	NAA10
NCBI Official Synonym Symbols:	TE2; ARD1; NATD; ARD1A; ARD1P; DXS707; MCOPS1
NCBI Protein Information:	N-alpha-acetyltransferase 10; natA catalytic subunit Naa10; ARD1 homolog A, N-acetyltransferase; N-acetyltransferase ARD1, human homolog of; N-alpha-acetyltransferase 10, NatA catalytic subunit; N-terminal acetyltransferase complex ARD1 subunit homolog A
UniProt Protein Name:	N-alpha-acetyltransferase 10
UniProt Synonym Protein Names:	N-terminal acetyltransferase complex ARD1 subunit homolog A; NatA catalytic subunit Naa10
Protein Family:	N-alpha-acetyltransferase
UniProt Gene Name:	NAA10
UniProt Entry Name:	NAA10_HUMAN

	Western blot analysis of extracts of various cell lines, using NAA10 antibody (CAB7009) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat lung using NAA10 antibody (CAB7009) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human breast cancer using NAA10 antibody (CAB7009) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using NAA10 antibody (CAB7009) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using NAA10 antibody (CAB7009) at dilution of 1:100 (40x lens).

Antibodies

NAA10 Antibody (PACO07803)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)