NAA60 Rabbit Polyclonal Antibody (CAB7386)

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SKU:
CAB7386
  • Western blot analysis of extracts of various cell lines, using NAA60 antibody (CAB7386) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
  • Immunohistochemistry of paraffin-embedded human liver cancer using NAA60 antibody (CAB7386) at dilution of 1:200 (40x lens).
  • Immunohistochemistry of paraffin-embedded human colon using NAA60 antibody (CAB7386) at dilution of 1:200 (40x lens).
  • Immunohistochemistry of paraffin-embedded human gastric using NAA60 antibody (CAB7386) at dilution of 1:200 (40x lens).
€99 - €249

Description

Technical Manual

NAA60 Rabbit Polyclonal Antibody

Product Name:NAA60 Rabbit Polyclonal Antibody
Product SKU:CAB7386
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

This gene encodes an enzyme that localizes to the Golgi apparatus, where it transfers an acetyl group to the N-terminus of free proteins. This enzyme acts on histones, and its activity is important for chromatin assembly and chromosome integrity. Alternative splicing and the use of alternative promoters results in multiple transcript variants. The upstream promoter is located in a differentially methylated region (DMR) and undergoes imprinting; transcript variants originating from this position are expressed from the maternal allele.

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-242 of human NAA60 (NP_079121.1).
Sequence:

MTEVVPSSALSEVSLRLLCHDDIDTVKHLCGDWFPIEYPDSWYRDITSNKKFFSLAATYRGAIVGMIVAEIKNRTKIHKEDGDILASNFSVDTQVAYILSLGVVKEFRKHGIGSLLLESLKDHISTTAQDHCKAIYLHVLTTNNTAINFYENRDFKQHHYLPYYYSIRGVLKDGFTYVLYINGGHPPWTILDYIQHLGSALASLSPCSIPHRVYRQAHSLLCSFLPWSGISSKSGIEYSRTM

Gene ID:79903
Swiss Prot:Q9H7X0
Synonyms:NAA60; HAT4; NAT15; NatF; hNaa60
Calculated MW:19kDa/20kDa/27kDa/28kDa
Observed MW:27kDa
Reactivity:Human, Mouse, Rat
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting Immunohistochemistry Immunofluorescence 
Positive Samples:Mouse brain, Rat brain
Cellular Location:Golgi apparatus membrane

Product Images

NAA60 Rabbit Polyclonal Antibody (CAB7386)

Western blot analysis of extracts of various cell lines, using NAA60 antibody (CAB7386) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.

NAA60 Rabbit Polyclonal Antibody (CAB7386)

Immunohistochemistry of paraffin-embedded human liver cancer using NAA60 antibody (CAB7386) at dilution of 1:200 (40x lens).

NAA60 Rabbit Polyclonal Antibody (CAB7386)

Immunohistochemistry of paraffin-embedded human colon using NAA60 antibody (CAB7386) at dilution of 1:200 (40x lens).

NAA60 Rabbit Polyclonal Antibody (CAB7386)

Immunohistochemistry of paraffin-embedded human gastric using NAA60 antibody (CAB7386) at dilution of 1:200 (40x lens).

Uniprot Information

UniProt Protein Function:NAT15: Histone acetyltransferase localized in the Golgi apparatus that mediates acetylation of free histone H4, thereby facilitating nucleosome assembly. Has a preference for free histone H4 'Lys-20'(H4K20ac), 'Lys-79'(H4K79ac) and 'Lys-91' (H4K91ac). Also displays alpha (N-terminal) acetyltransferase activity towards a range of N-terminal sequences including those starting with Met-Lys, Met-Val, Met-Ala and Met-Met. Required for normal chromosomal segregation during anaphase. Belongs to the acetyltransferase family. NAA60 subfamily. 5 isoforms of the human protein are produced by alternative promoter.
UniProt Protein Details:

Protein type:EC 2.3.1.48; Acetyltransferase; EC 2.3.1.88

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: Golgi membrane

Molecular Function:peptide alpha-N-acetyltransferase activity; H4 histone acetyltransferase activity

Biological Process: nucleosome assembly; cell proliferation; N-terminal peptidyl-methionine acetylation; chromosome segregation

UniProt Code:Q9H7X0
NCBI GenInfo Identifier:74733721
NCBI Gene ID:79903
NCBI Accession:Q9H7X0.1
UniProt Secondary Accession:Q9H7X0,Q6IA31, Q6UX26, B3KRQ0, B4DLZ0, B4DPZ8, B4DYC4 D3DUC2, E7EQ65,
UniProt Related Accession:Q9H7X0
Molecular Weight:242
NCBI Full Name:N-alpha-acetyltransferase 60
NCBI Synonym Full Names:N(alpha)-acetyltransferase 60, NatF catalytic subunit
NCBI Official Symbol:NAA60 
NCBI Official Synonym Symbols:HAT4; NAT15 
NCBI Protein Information:N-alpha-acetyltransferase 60; natF catalytic subunit; histone acetyltransferase type B protein 4; N-acetyltransferase 15 (GCN5-related, putative)
UniProt Protein Name:N-alpha-acetyltransferase 60
UniProt Synonym Protein Names:Histone acetyltransferase type B protein 4; HAT4; N-acetyltransferase 15; NatF catalytic subunit
Protein Family:N-alpha-acetyltransferase
UniProt Gene Name:NAA60 
UniProt Entry Name:NAA60_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
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