NAA60 Rabbit Polyclonal Antibody (CAB7386)
- SKU:
- CAB7386
Description
NAA60 Rabbit Polyclonal Antibody
| Product Name: | NAA60 Rabbit Polyclonal Antibody |
| Product SKU: | CAB7386 |
| Size: | 20uL, 50 uL |
| Host Species: | Rabbit |
| Purification Method: | Affinity purification |
| Isotype: | IgG |
| Background: | This gene encodes an enzyme that localizes to the Golgi apparatus, where it transfers an acetyl group to the N-terminus of free proteins. This enzyme acts on histones, and its activity is important for chromatin assembly and chromosome integrity. Alternative splicing and the use of alternative promoters results in multiple transcript variants. The upstream promoter is located in a differentially methylated region (DMR) and undergoes imprinting; transcript variants originating from this position are expressed from the maternal allele. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-242 of human NAA60 (NP_079121.1). |
| Sequence: | MTEVVPSSALSEVSLRLLCHDDIDTVKHLCGDWFPIEYPDSWYRDITSNKKFFSLAATYRGAIVGMIVAEIKNRTKIHKEDGDILASNFSVDTQVAYILSLGVVKEFRKHGIGSLLLESLKDHISTTAQDHCKAIYLHVLTTNNTAINFYENRDFKQHHYLPYYYSIRGVLKDGFTYVLYINGGHPPWTILDYIQHLGSALASLSPCSIPHRVYRQAHSLLCSFLPWSGISSKSGIEYSRTM |
| Gene ID: | 79903 |
| Swiss Prot: | Q9H7X0 |
| Synonyms: | NAA60; HAT4; NAT15; NatF; hNaa60 |
| Calculated MW: | 19kDa/20kDa/27kDa/28kDa |
| Observed MW: | 27kDa |
| Reactivity: | Human, Mouse, Rat |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Application Key: | Western blotting Immunohistochemistry Immunofluorescence |
| Positive Samples: | Mouse brain, Rat brain |
| Cellular Location: | Golgi apparatus membrane |
Product Images
| Western blot analysis of extracts of various cell lines, using NAA60 antibody (CAB7386) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s. |
| Immunohistochemistry of paraffin-embedded human liver cancer using NAA60 antibody (CAB7386) at dilution of 1:200 (40x lens). |
| Immunohistochemistry of paraffin-embedded human colon using NAA60 antibody (CAB7386) at dilution of 1:200 (40x lens). |
| Immunohistochemistry of paraffin-embedded human gastric using NAA60 antibody (CAB7386) at dilution of 1:200 (40x lens). |
Uniprot Information
| UniProt Protein Function: | NAT15: Histone acetyltransferase localized in the Golgi apparatus that mediates acetylation of free histone H4, thereby facilitating nucleosome assembly. Has a preference for free histone H4 'Lys-20'(H4K20ac), 'Lys-79'(H4K79ac) and 'Lys-91' (H4K91ac). Also displays alpha (N-terminal) acetyltransferase activity towards a range of N-terminal sequences including those starting with Met-Lys, Met-Val, Met-Ala and Met-Met. Required for normal chromosomal segregation during anaphase. Belongs to the acetyltransferase family. NAA60 subfamily. 5 isoforms of the human protein are produced by alternative promoter. |
| UniProt Protein Details: | Protein type:EC 2.3.1.48; Acetyltransferase; EC 2.3.1.88 Chromosomal Location of Human Ortholog: 16p13.3 Cellular Component: Golgi membrane Molecular Function:peptide alpha-N-acetyltransferase activity; H4 histone acetyltransferase activity Biological Process: nucleosome assembly; cell proliferation; N-terminal peptidyl-methionine acetylation; chromosome segregation |
| UniProt Code: | Q9H7X0 |
| NCBI GenInfo Identifier: | 74733721 |
| NCBI Gene ID: | 79903 |
| NCBI Accession: | Q9H7X0.1 |
| UniProt Secondary Accession: | Q9H7X0,Q6IA31, Q6UX26, B3KRQ0, B4DLZ0, B4DPZ8, B4DYC4 D3DUC2, E7EQ65, |
| UniProt Related Accession: | Q9H7X0 |
| Molecular Weight: | 242 |
| NCBI Full Name: | N-alpha-acetyltransferase 60 |
| NCBI Synonym Full Names: | N(alpha)-acetyltransferase 60, NatF catalytic subunit |
| NCBI Official Symbol: | NAA60 |
| NCBI Official Synonym Symbols: | HAT4; NAT15 |
| NCBI Protein Information: | N-alpha-acetyltransferase 60; natF catalytic subunit; histone acetyltransferase type B protein 4; N-acetyltransferase 15 (GCN5-related, putative) |
| UniProt Protein Name: | N-alpha-acetyltransferase 60 |
| UniProt Synonym Protein Names: | Histone acetyltransferase type B protein 4; HAT4; N-acetyltransferase 15; NatF catalytic subunit |
| Protein Family: | N-alpha-acetyltransferase |
| UniProt Gene Name: | NAA60 |
| UniProt Entry Name: | NAA60_HUMAN |
Additional Information
Product type: |
Antibody |
Reactivity: |
Human |
Reactivity: |
Mouse |
Reactivity: |
Rat |
Host Species: |
Rabbit |
Isotype: |
IgG |
Antibody Type: |
Polyclonal Antibody |
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