Anti-NCSTN Antibody (CAB14505)

Antibody Name:	Anti-NCSTN Antibody
Antibody SKU:	CAB14505
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 429-669 of human NCSTN (NP_056146.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse brain, Rat brain, Rat spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 429-669 of human NCSTN (NP_056146.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	RFLR ARNI SGVV LADH SGAF HNKY YQSI YDTA ENIN VSYP EWLS PEED LNFV TDTA KALA DVAT VLGR ALYE LAGG TNFS DTVQ ADPQ TVTR LLYG FLIK ANNS WFQS ILRQ DLRS YLGD GPLQ HYIA VSSP TNTT YVVQ YALA NLTG TVVN LTRE QCQD PSKV PSEN KDLY EYSW VQGP LHSN ETDR LPRC VRST ARLA RALS PAFE LSQW SSTE YSTW TESR WKDI RARI FLIA SKEL E
Gene ID:	23385
Uniprot:	Q92542
Cellular Location:	Melanosome, Membrane, Single-pass type I membrane protein
Calculated MW:	76kDa/78kDa
Observed MW:	110kDa

Synonyms:

NCSTN, ATAG1874, nicastrin

Background:

This gene encodes a type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex. The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly. The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease; however, the nature of the encoded protein's role in Alzheimer's disease is not known for certain. Mutations in this gene are associated with familial acne inversa. A pseudogene of this gene is present on chromosome 21. Alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

UniProt Protein Function:	nicastrin: Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta- amyloid precursor protein). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex. Component of the gamma-secretase complex, a complex composed of a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PEN2. Such minimal complex is sufficient for secretase activity, although other components may exist. Binds to proteolytic processed C-terminal fragments C83 and C99 of the amyloid precursor protein (APP). Constitutively expressed in neural cells. Widely expressed. Belongs to the nicastrin family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 1q22-q23 Cellular Component: Golgi apparatus; focal adhesion; membrane; lysosomal membrane; integral to plasma membrane; endoplasmic reticulum; melanosome; plasma membrane; integral to membrane Molecular Function:protein binding; endopeptidase activity Biological Process: positive regulation of catalytic activity; axon guidance; extracellular matrix organization and biogenesis; Notch signaling pathway; membrane protein intracellular domain proteolysis; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; membrane protein ectodomain proteolysis; myeloid cell homeostasis; Notch receptor processing; proteolysis; T cell proliferation; extracellular matrix disassembly; epithelial cell proliferation; amyloid precursor protein catabolic process; beta-amyloid metabolic process; ephrin receptor signaling pathway; protein processing Disease: Acne Inversa, Familial, 1
NCBI Summary:	This gene encodes a type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex. The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly. The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease; however, the nature of the encoded protein's role in Alzheimer's disease is not known for certain. Mutations in this gene are associated with familial acne inversa. A pseudogene of this gene is present on chromosome 21. Alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Feb 2014]
UniProt Code:	Q92542
NCBI GenInfo Identifier:	12231037
NCBI Gene ID:	23385
NCBI Accession:	Q92542.2
UniProt Secondary Accession:	Q92542,Q5T207, Q5T208, Q86VV5,
UniProt Related Accession:	Q92542
Molecular Weight:	709
NCBI Full Name:	Nicastrin
NCBI Synonym Full Names:	nicastrin
NCBI Official Symbol:	NCSTN
NCBI Official Synonym Symbols:	ATAG1874
NCBI Protein Information:	nicastrin; anterior pharynx-defective 2
UniProt Protein Name:	Nicastrin
Protein Family:	Nicastrin
UniProt Gene Name:	NCSTN
UniProt Entry Name:	NICA_HUMAN

	Western blot analysis of extracts of various cell lines, using NCSTN antibody (CAB14505) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunofluorescence analysis of L929 cells using NCSTN Rabbit pAb (CAB14505) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U2OS cells using NCSTN Rabbit pAb (CAB14505) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-NCSTN Antibody (CAB0128)

Anti-NCSTN Antibody (CAB17111)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)