Anti-NDE1 Antibody (CAB7112)

Antibody Name:	Anti-NDE1 Antibody
Antibody SKU:	CAB7112
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human NDE1 (NP_060138.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, HepG2, SKOV3, MCF7, A673, Mouse heart, Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human NDE1 (NP_060138.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEDS GKTF SSEE EEAN YWKD LAMT YKQR AENT QEEL REFQ EGSR EYEA ELET QLQQ IETR NRDL LSEN NRLR MELE TIKE KFEV QHSE GYRQ ISAL EDDL AQTK AIKD QL
Gene ID:	54820
Uniprot:	Q9NXR1
Cellular Location:	Chromosome, Cleavage furrow, Cytoplasm, centromere, centrosome, cytoskeleton, kinetochore, microtubule organizing center, spindle
Calculated MW:	37kDa/38kDa
Observed MW:	39kDa

Synonyms:

NDE1, HOM-TES-87, LIS4, MHAC, NDE, NUDE, NUDE1

Background:

This gene encodes a member of the nuclear distribution E (NudE) family of proteins. The encoded protein is localized at the centrosome and interacts with other centrosome components as part of a multiprotein complex that regulates dynein function. This protein plays an essential role in microtubule organization, mitosis and neuronal migration. Mutations in this gene cause lissencephaly 4, a disorder characterized by lissencephaly, severe brain atrophy, microcephaly, and severe mental retardation. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	NUDE1: a microtubule-associated protein required for centrosome duplication, and the formation and function of the mitotic spindle. Associated with the centrosome, kinetochore, and spindle. Specifically phosphorylated during M phase, inducing its association with mitotic spindles. Appears to participate in dynein-mediated transport of kinetochore proteins to spindle poles along microtubules. Interacts with gamma tubulin, dynactin, CEP110, dynein,TCP1. PAFAH1B1, PCM-1 and PCNT. Concentrates at the plus ends of microtubules coincident with kinetochores in metaphase and anaphase, and at the cleavage furrow during cytokinesis.Two alternatively spliced human isoforms have been described.
UniProt Protein Details:	Protein type:Microtubule-binding; Cell cycle regulation; Cytoskeletal Chromosomal Location of Human Ortholog: 16p13.11 Cellular Component: kinetochore; centrosome; kinesin complex; microtubule; membrane; spindle pole centrosome; cytosol; cleavage furrow Molecular Function:protein domain specific binding; identical protein binding; protein binding; microtubule binding Biological Process: cell migration; organelle organization and biogenesis; establishment of chromosome localization; neuron migration; microtubule nucleation; chromosome segregation; mitotic centrosome separation; centrosome localization; establishment of mitotic spindle orientation; cell division; neuroblast proliferation; vesicle transport along microtubule; mitotic cell cycle; cerebral cortex development; G2/M transition of mitotic cell cycle; centrosome duplication Disease: Lissencephaly 4; Microhydranencephaly
UniProt Code:	Q9NXR1
NCBI GenInfo Identifier:	108860813
NCBI Gene ID:
NCBI Accession:	Q9NXR1.2
Molecular Weight:	37kDa; 38kDa
NCBI Full Name:	Nuclear distribution protein nudE homolog 1
UniProt Protein Name:	Nuclear distribution protein nudE homolog 1
Protein Family:	Nuclear distribution protein
UniProt Gene Name:	NDE1
UniProt Entry Name:	NDE1_HUMAN

	Western blot analysis of extracts of various cell lines, using NDE1 antibody (CAB7112) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat brain using NDE1 antibody (CAB7112) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat spleen using NDE1 antibody (CAB7112) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human breast cancer using NDE1 antibody (CAB7112) at dilution of 1:100 (40x lens).

Antibodies

Anti-NDE1 Antibody (CAB14136)

NDE1 Antibody (PACO10768)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)