The NDUFB10 Antibody (CAB3982) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, has high reactivity with human samples and has been validated for use in Western blot applications.The NDUFB10 protein plays a crucial role in mitochondrial function, specifically in electron transport and ATP production. Dysregulation of complex I subunits like NDUFB10 has been implicated in various diseases, including neurodegenerative disorders and metabolic syndromes. By using the NDUFB10 Polyclonal Antibody, researchers can detect and analyze NDUFB10 expression in different cell types, providing insights into mitochondrial dysfunction and potential therapeutic targets.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NDUFB10 Antibody
SKU:
CAB3982
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Involved in mitochondrial respiratory chain complex I assembly. Located in mitochondrial inner membrane. Part of mitochondrial respiratory chain complex I. Implicated in nuclear type mitochondrial complex I deficiency 35.
Purification Method
Affinity purification
Gene ID
4716
RRID
AB_2765427
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using NDUFB10 Rabbit pAb (CAB3982) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg NDUFB10 antibody (CAB3982). Western blot was performed from the immunoprecipitate using NDUFB10 antibody (CAB3982) at a dilution of 1:1000.
