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Anti-NDUFB10 Antibody (CAB3982)

SKU:
CAB3982
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
  • Cell Biology Antibodies 9 Anti-NDUFB10 Antibody CAB3982
  • Cell Biology Antibodies 9 Anti-NDUFB10 Antibody CAB3982
  • Cell Biology Antibodies 9 Anti-NDUFB10 Antibody CAB3982
  • Cell Biology Antibodies 9 Anti-NDUFB10 Antibody CAB3982
  • Cell Biology Antibodies 9 Anti-NDUFB10 Antibody CAB3982
€139 - €419
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Description

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Antibody Name:Anti-NDUFB10 Antibody
Antibody SKU:CAB3982
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human NDUFB10 (NP_004539.1).
Application:WB IHC IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IP 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2, HeLa, MCF-7, Jurkat, Mouse brain, Mouse kidney, Mouse heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human NDUFB10 (NP_004539.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPDS WDKD VYPE PPRR TPVQ PNPI VYMM KAFD LIVD RPVT LVRE FIER QHAK NRYY YYHR QYRR VPDI TECK EEDI MCMY EAEM QWKR DYKV DQEI INIM QDRL KACQ QREG QNYQ QNCI KEVE QFTQ VAKA YQDR YQDL GAYS SARK CLAK QRQR MLQE RKAA KEAA AATS
Gene ID:4716
Uniprot:O96000
Cellular Location:Matrix side, Mitochondrion inner membrane, Peripheral membrane protein
Calculated MW:20kDa
Observed MW:21kDa
Synonyms:NDUFB10, PDSW
Background:
UniProt Protein Function:NDUFB10: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I NDUFB10 subunit family.
UniProt Protein Details:

Protein type:Oxidoreductase; Mitochondrial; EC 1.6.5.3; Energy Metabolism - oxidative phosphorylation; EC 1.6.99.3

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: mitochondrial inner membrane; mitochondrial respiratory chain complex I

Molecular Function:protein binding; NADH dehydrogenase (ubiquinone) activity

Biological Process: cellular metabolic process; mitochondrial electron transport, NADH to ubiquinone

UniProt Code:O96000
NCBI GenInfo Identifier:6093491
NCBI Gene ID:4716
NCBI Accession:O96000.3
UniProt Secondary Accession:O96000,Q96II6,
UniProt Related Accession:O96000
Molecular Weight:172
NCBI Full Name:NADH dehydrogenase
NCBI Synonym Full Names:NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 10, 22kDa
NCBI Official Symbol:NDUFB10  
NCBI Official Synonym Symbols:PDSW  
NCBI Protein Information:NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10; CI-PDSW; complex I-PDSW; complex I PDSW subunit; NADH-ubiquinone oxidoreductase PDSW subunit; NADH ubiquinone oxidoreductase PDSW subunit (RH 16p13.3)
UniProt Protein Name:NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10
UniProt Synonym Protein Names:Complex I-PDSW; CI-PDSW; NADH-ubiquinone oxidoreductase PDSW subunit
UniProt Gene Name:NDUFB10  
UniProt Entry Name:NDUBA_HUMAN
Anti-NDUFB10 Antibody (CAB3982)Western blot analysis of extracts of various cell lines, using NDUFB10 antibody (CAB3982) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-NDUFB10 Antibody (CAB3982)Immunohistochemistry of paraffin-embedded mouse stomach using NDUFB10 antibody (CAB3982) at dilution of 1:100 (40x lens).
Anti-NDUFB10 Antibody (CAB3982)Immunohistochemistry of paraffin-embedded rat kidney using NDUFB10 antibody (CAB3982) at dilution of 1:100 (40x lens).
Anti-NDUFB10 Antibody (CAB3982)Immunohistochemistry of paraffin-embedded human stomach using NDUFB10 antibody (CAB3982) at dilution of 1:100 (40x lens).
Anti-NDUFB10 Antibody (CAB3982)Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug NDUFB10 antibody (CAB3982). Western blot was performed from the immunoprecipitate using NDUFB10 antibody (CAB3982) at a dilition of 1:1000.

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