Anti-NDUFB7 Antibody (CAB13693)

Antibody Name:	Anti-NDUFB7 Antibody
Antibody SKU:	CAB13693
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-137 of human NDUFB7 (NP_004137.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-431, HL-60, MCF7, Mouse brain, Mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-137 of human NDUFB7 (NP_004137.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGAH LVRR YLGD ASVE PDPL QMPT FPPD YGFP ERKE REMV ATQQ EMMD AQLR LQLR DYCA HHLI RLLK CKRD SFPN FLAC KQER HDWD YCEH RDYV MRMK EFER ERRL LQRK KRRE KKAA ELAK GQGP GEVD PKVA L
Gene ID:	4713
Uniprot:	P17568
Cellular Location:	Mitochondrion inner membrane, Mitochondrion intermembrane space, Peripheral membrane protein
Calculated MW:	16kDa
Observed MW:	16kDa

Synonyms:

NDUFB7, B18, CI-B18

Background:

The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It is located at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

UniProt Protein Function:	NDUFB7: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I NDUFB7 subunit family.
UniProt Protein Details:	Protein type:EC 1.6.99.3; EC 1.6.5.3; Oxidoreductase; Mitochondrial; Energy Metabolism - oxidative phosphorylation Chromosomal Location of Human Ortholog: 19p13.12 Cellular Component: mitochondrial inner membrane; mitochondrial intermembrane space; mitochondrial respiratory chain complex I; mitochondrion Biological Process: mitochondrial electron transport, NADH to ubiquinone; mitochondrial respiratory chain complex I assembly
NCBI Summary:	The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It is located at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [provided by RefSeq, Jul 2008]
UniProt Code:	P17568
NCBI GenInfo Identifier:	12644140
NCBI Gene ID:	4713
NCBI Accession:	P17568.4
UniProt Secondary Accession:	P17568,Q6ICN9, Q9UI16,
UniProt Related Accession:	P17568
Molecular Weight:	16,402 Da
NCBI Full Name:	NADH dehydrogenase
NCBI Synonym Full Names:	NADH:ubiquinone oxidoreductase subunit B7
NCBI Official Symbol:	NDUFB7
NCBI Official Synonym Symbols:	B18; CI-B18
NCBI Protein Information:	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7
UniProt Protein Name:	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7
UniProt Synonym Protein Names:	Cell adhesion protein SQM1; Complex I-B18; CI-B18; NADH-ubiquinone oxidoreductase B18 subunit
Protein Family:	NADH dehydrogenase
UniProt Gene Name:	NDUFB7
UniProt Entry Name:	NDUB7_HUMAN

	Western blot analysis of extracts of various cell lines, using NDUFB7 antibody (CAB13693) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat heart using NDUFB7 antibody (CAB13693) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophageal using NDUFB7 antibody (CAB13693) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spleen using NDUFB7 antibody (CAB13693) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of L929 cells using NDUFB7 Polyclonal Antibody (CAB13693) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

NDUFB7 Antibody (PACO10790)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)