Anti-NDUFB9 Antibody (CAB17454)

Antibody Name:	Anti-NDUFB9 Antibody
Antibody SKU:	CAB17454
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic Peptide of human NDUFB9.

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, HepG2, HeLa, Mouse brain, Rat kidney, Rat heart, Rat brain

Immunogen:	A synthetic Peptide of human NDUFB9.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	4715
Uniprot:	Q9Y6M9
Cellular Location:	Matrix side, Mitochondrion inner membrane, Peripheral membrane protein
Calculated MW:	21kDa
Observed MW:	22kDa

Synonyms:

NDUFB9, B22, CI-B22, LYRM3, UQOR22

Background:

The protein encoded by this gene is a subunit of the mitochondrial oxidative phosphorylation complex I (nicotinamide adenine dinucleotide: ubiquinone oxidoreductase). Complex I is localized to the inner mitochondrial membrane and functions to dehydrogenate nicotinamide adenine dinucleotide and to shuttle electrons to coenzyme Q. Complex I deficiency is the most common defect found in oxidative phosphorylation disorders and results in a range of conditions, including lethal neonatal disease, hypertrophic cardiomyopathy, liver disease, and adult-onset neurodegenerative disorders. Pseudogenes of this gene are found on chromosomes five, seven and eight. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	NDUFB9: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I LYR family.
UniProt Protein Details:	Protein type:Mitochondrial; EC 1.6.5.3; Oxidoreductase; Energy Metabolism - oxidative phosphorylation; EC 1.6.99.3 Chromosomal Location of Human Ortholog: 8q13.3 Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial respiratory chain complex I Molecular Function:NADH dehydrogenase (ubiquinone) activity Biological Process: cellular metabolic process; sensory perception of sound; mitochondrial electron transport, NADH to ubiquinone Disease: Mitochondrial Complex I Deficiency
NCBI Summary:	The protein encoded by this gene is a subunit of the mitochondrial oxidative phosphorylation complex I (nicotinamide adenine dinucleotide: ubiquinone oxidoreductase). Complex I is localized to the inner mitochondrial membrane and functions to dehydrogenate nicotinamide adenine dinucleotide and to shuttle electrons to coenzyme Q. Complex I deficiency is the most common defect found in oxidative phosphorylation disorders and results in a range of conditions, including lethal neonatal disease, hypertrophic cardiomyopathy, liver disease, and adult-onset neurodegenerative disorders. Pseudogenes of this gene are found on chromosomes five, seven and eight. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]
UniProt Code:	Q9Y6M9
NCBI GenInfo Identifier:	8134589
NCBI Gene ID:	4715
NCBI Accession:	Q9Y6M9.3
UniProt Secondary Accession:	Q9Y6M9,Q9UQE8, B2R8M6,
UniProt Related Accession:	Q9Y6M9
Molecular Weight:	21,831 Da
NCBI Full Name:	NADH dehydrogenase
NCBI Synonym Full Names:	NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa
NCBI Official Symbol:	NDUFB9
NCBI Official Synonym Symbols:	B22; LYRM3; CI-B22; UQOR22
NCBI Protein Information:	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9; complex I-B22; complex I B22 subunit; LYR motif-containing protein 3; NADH-ubiquinone oxidoreductase B22 subunit
UniProt Protein Name:	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9
UniProt Synonym Protein Names:	Complex I-B22; CI-B22; LYR motif-containing protein 3; NADH-ubiquinone oxidoreductase B22 subunit
Protein Family:	NADH dehydrogenase
UniProt Gene Name:	NDUFB9
UniProt Entry Name:	NDUB9_HUMAN

	Western blot analysis of extracts of various cell lines, using NDUFB9 Rabbit pAb (CAB17454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded rat heart using NDUFB9 antibody (CAB17454) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon using NDUFB9 antibody (CAB17454) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse pancreas using NDUFB9 antibody (CAB17454) at dilution of 1:100 (40x lens).

Antibodies

NDUFB9 Antibody (PACO06674)

NDUFB9 Antibody (PACO10792)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)