Anti-Neurocalcin-delta Antibody (CAB8000)

Antibody Name:	NCALD Rabbit Polyclonal Antibody
Antibody SKU:	CAB8000
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human NCALD (NP_114430.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, THP-1, BT-474, Mouse brain, Mouse liver, Mouse lung, Mouse spinal cord, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human NCALD (NP_114430.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGKQ NSKL RPEV MQDL LEST DFTE HEIQ EWYK GFLR DCPS GHLS MEEF KKIY GNFF PYGD ASKF AEHV FRTF DANG DGTI DFRE FIIA LSVT SRGK LEQK LKWA FSMY DLDG NGYI SKAE MLEI VQAI YKMV SSVM KMPE DEST PEKR TEKI FRQM DTNR DGKL SLEE FIRG AKSD PSIV RLLQ CDPS SAGQ F
Gene ID:	83988
Uniprot:	P61601
Cellular Location:
Calculated MW:	22kDa
Observed MW:	22kDa

Synonyms:

NCALD

Background:

This gene encodes a member of the neuronal calcium sensor (NCS) family of calcium-binding proteins. The protein contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. The protein is cytosolic at resting calcium levels; however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. The protein is thought to be a regulator of G protein-coupled receptor signal transduction. Several alternatively spliced variants of this gene have been determined, all of which encode the same protein; additional variants may exist but their biological validity has not been determined.

UniProt Protein Function:	NCALD: May be involved in the calcium-dependent regulation of rhodopsin phosphorylation. Binds three calcium ions. Belongs to the recoverin family.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 8q22.2 Cellular Component: clathrin coat of trans-Golgi network vesicle; intracellular; cytosol Molecular Function:tubulin binding; protein binding; clathrin binding; calcium ion binding; actin binding; alpha-tubulin binding Biological Process: vesicle-mediated transport; synaptic transmission; regulation of systemic arterial blood pressure; calcium-mediated signaling
NCBI Summary:	This gene encodes a member of the neuronal calcium sensor (NCS) family of calcium-binding proteins. The protein contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. The protein is cytosolic at resting calcium levels; however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. The protein is thought to be a regulator of G protein-coupled receptor signal transduction. Several alternatively spliced variants of this gene have been determined, all of which encode the same protein; additional variants may exist but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
UniProt Code:	P61601
NCBI GenInfo Identifier:	47606440
NCBI Gene ID:	83988
NCBI Accession:	P61601.2
UniProt Related Accession:	P61601
Molecular Weight:
NCBI Full Name:	Neurocalcin-delta
NCBI Synonym Full Names:	neurocalcin delta
NCBI Official Symbol:	NCALD
NCBI Protein Information:	neurocalcin-delta
UniProt Protein Name:	Neurocalcin-delta
Protein Family:	Neurocalcin
UniProt Gene Name:	NCALD
UniProt Entry Name:	NCALD_HUMAN

	Western blot analysis of extracts of various cell lines, using NCALD antibody (CAB8000) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of A549 cells using NCALD antibody (CAB8000). Blue: DAPI for nuclear staining.

Antibodies

NCALD Antibody (PACO05487)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)