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Western blot analysis of extracts of mouse brain, using NeuroD1 Rabbit mAb (CAB12020) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Key Features

NeuroD1 Monoclonal Antibody (CAB12020)

Datasheet MSDS
SKU CAB12020
Product Type Antibody
Antibody Type Monoclonal Antibody
Reactivity Mouse
Host Species Rabbit
Synonyms T2D, BETA2, BHF-1, MODY6, NEUROD, bHLHa3, NeuroD1
$173 - $603

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Description

system_update_altDatasheetsystem_update_altMSDS

NeuroD1 Monoclonal Antibody (CAB12020)

The NeuroD1 Monoclonal Antibody (CAB12020) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is specifically designed to target NeuroD1 and is highly reactive with human samples, making it ideal for use in various research applications like immunohistochemistry and flow cytometry. By binding to NeuroD1, this antibody enables the detection and analysis of NeuroD1 protein levels in different cell types, providing valuable insights into neurogenesis and neuronal development.NeuroD1, also known as Neurogenic Differentiation 1, plays a key role in neuronal differentiation and maturation, making it a key player in neurodevelopmental processes.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.

Product Name:NeuroD1 Monoclonal Antibody
SKU:CAB12020
Size:20μL, 100μL
Reactivity:Mouse
Clone Number:ARC0483
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:MTKS YSES GLMG EPQP QGPP SWTD ECLS SQDE EHEA DKKE DDLE TMNA EEDS LRNG GEEE DEDE DLEE EEEE EEED DDQK PKRR GPKK KKMT KARL ERFK
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:1000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:T2D, BETA2, BHF-1, MODY6, NEUROD, bHLHa3, NeuroD1
Positive Sample:Mouse brain
Cellular Localization:Cytoplasm, Nucleoplasm, Nucleus.
Calculated MW:40kDa
Observed MW:49kDa

This gene encodes a member of the NeuroD family of basic helix-loop-helix (bHLH) transcription factors. The protein forms heterodimers with other bHLH proteins and activates transcription of genes that contain a specific DNA sequence known as the E-box. It regulates expression of the insulin gene, and mutations in this gene result in type II diabetes mellitus.

Purification MethodAffinity purification
Gene ID4760
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from mouse brain, using NeuroD1 Rabbit mAb (CAB12020) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
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