Anti-NRP2 Antibody (CAB2581)

Antibody Name:	Anti-NRP2 Antibody
Antibody SKU:	CAB2581
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 150-420 of human NRP2 (NP_003863.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	A-549, HT-1080, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 150-420 of human NRP2 (NP_003863.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SKNF TSPN GTIE SPGF PEKY PHNL DCTF TILA KPKM EIIL QFLI FDLE HDPL QVGE GDCK YDWL DIWD GIPH VGPL IGKY CGTK TPSE LRSS TGIL SLTF HTDM AVAK DGFS ARYY LVHQ EPLE NFQC NVPL GMES GRIA NEQI SASS TYSD GRWT PQQS RLHG DDNG WTPN LDSN KEYL QVDL RFLT MLTA IATQ GAIS RETQ NGYY VKSY KLEV STNG EDWM VYRH GKNH KVFQ ANND ATEV VLNK LHAP LLTR FVRI RPQT WHSG IAL
Gene ID:	8828
Uniprot:	O60462
Cellular Location:	Membrane, Secreted, Single-pass type I membrane protein
Calculated MW:	62kDa/101kDa/102kDa/104kDa
Observed MW:	105kDa/128kDa

Synonyms:

NRP2, NP2, NPN2, PRO2714, VEGF165R2, neuropilin-2

Background:

This gene encodes a member of the neuropilin family of receptor proteins. The encoded transmembrane protein binds to SEMA3C protein {sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C} and SEMA3F protein {sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F}, and interacts with vascular endothelial growth factor (VEGF). This protein may play a role in cardiovascular development, axon guidance, and tumorigenesis. Multiple transcript variants encoding distinct isoforms have been identified for this gene.

UniProt Protein Function:	NRP2: High affinity receptor for semaphorins 3C, 3F, VEGF-165 and VEGF-145 isoforms of VEGF, and the PLGF-2 isoform of PGF. Heterodimer with NRP1. Binds PLXNB1. Belongs to the neuropilin family. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral; Receptor, misc.; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 2q33.3 Cellular Component: extracellular region; integral to membrane; membrane; plasma membrane Molecular Function:cytokine binding; growth factor binding; heparin binding; metal ion binding; receptor activity; semaphorin receptor activity; vascular endothelial growth factor receptor activity Biological Process: angiogenesis; axon extension involved in axon guidance; axon guidance; cell adhesion; nerve development; positive regulation of endothelial cell proliferation; vascular endothelial growth factor receptor signaling pathway
NCBI Summary:	This gene encodes a member of the neuropilin family of receptor proteins. The encoded transmembrane protein binds to SEMA3C protein {sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C} and SEMA3F protein {sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F}, and interacts with vascular endothelial growth factor (VEGF). This protein may play a role in cardiovascular development, axon guidance, and tumorigenesis. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	O60462
NCBI GenInfo Identifier:	215274096
NCBI Gene ID:	8828
NCBI Accession:	O60462.2
UniProt Secondary Accession:	O60462,O14820, O14821, Q53TQ4, Q53TS3, Q7LBX7, Q9H2D4 Q9H2D5, Q9H2E2, Q9H2E3, Q9H2E4, E9PF66,
UniProt Related Accession:	O60462
Molecular Weight:	62,458 Da
NCBI Full Name:	Neuropilin-2
NCBI Synonym Full Names:	neuropilin 2
NCBI Official Symbol:	NRP2
NCBI Official Synonym Symbols:	NP2; NPN2; PRO2714; VEGF165R2
NCBI Protein Information:	neuropilin-2
UniProt Protein Name:	Neuropilin-2
UniProt Synonym Protein Names:	Vascular endothelial cell growth factor 165 receptor 2
Protein Family:	Neuropilin
UniProt Gene Name:	NRP2
UniProt Entry Name:	NRP2_HUMAN

Western blot analysis of extracts of various cell lines, using NRP2 antibody (CAB2581) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.

Antibodies

Anti-NRP2 Antibody (CAB12758)

NRP2 Antibody (PACO01941)

NRP2 Antibody (PACO03165)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)