Anti-NTF3 Antibody (CAB3179)

Antibody Name:	Anti-NTF3 Antibody
Antibody SKU:	CAB3179
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant protein of human NTF3

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:100 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	Mouse heart

Immunogen:	Recombinant protein of human NTF3
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	4908
Uniprot:	P20783
Cellular Location:	Secreted
Calculated MW:	29kDa/30kDa
Observed MW:	29kDa

Synonyms:

NTF3, HDNF, NGF-2, NGF2, NT-3, NT3

Background:

The protein encoded by this gene is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse.

UniProt Protein Function:	NT-3: Seems to promote the survival of visceral and proprioceptive sensory neurons. Belongs to the NGF-beta family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cytokine; Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 12p13 Cellular Component: cytoplasmic membrane-bound vesicle; extracellular region Molecular Function:neurotrophin p75 receptor binding; growth factor activity; nerve growth factor binding; receptor binding; chemoattractant activity Biological Process: myelination; axon guidance; mechanoreceptor differentiation; epidermis development; glial cell fate determination; activation of MAPK activity; regulation of neuron differentiation; positive regulation of receptor internalization; positive regulation of glial cell differentiation; signal transduction; enteric nervous system development; induction of positive chemotaxis; positive chemotaxis; cell-cell signaling; positive regulation of cell proliferation; negative regulation of neuron apoptosis; regulation of synaptic transmission; negative regulation of peptidyl-tyrosine phosphorylation; nervous system development; activation of protein kinase B; positive regulation of peptidyl-serine phosphorylation; peripheral nervous system development; positive regulation of peptidyl-tyrosine phosphorylation; neuromuscular synaptic transmission; nerve development; smooth muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; brain development; transmembrane receptor protein tyrosine kinase signaling pathway; neurite morphogenesis; positive regulation of cell migration
NCBI Summary:	The protein encoded by this gene is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. [provided by RefSeq, Jul 2008]
UniProt Code:	P20783
NCBI GenInfo Identifier:	128581
NCBI Gene ID:	4908
NCBI Accession:	P20783.1
UniProt Secondary Accession:	P20783,Q6FH50, B7Z1T5,
UniProt Related Accession:	P20783
Molecular Weight:	30,800 Da
NCBI Full Name:	Neurotrophin-3
NCBI Synonym Full Names:	neurotrophin 3
NCBI Official Symbol:	NTF3
NCBI Official Synonym Symbols:	NT3; HDNF; NGF2; NT-3; NGF-2
NCBI Protein Information:	neurotrophin-3; neurotrophic factor; nerve growth factor 2
UniProt Protein Name:	Neurotrophin-3
UniProt Synonym Protein Names:	HDNF; Nerve growth factor 2; NGF-2; Neurotrophic factor
Protein Family:	Neurotrophin
UniProt Gene Name:	NTF3
UniProt Entry Name:	NTF3_HUMAN

	Western blot analysis of extracts of mouse heart, using NTF3 antibody (CAB3179) at 1:250 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded human esophagus using NTF3 antibody (CAB3179) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using NTF3 antibody (CAB3179) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-NTF3 Antibody (CAB12475)	Human NT-3 (Neurotrophin-3) CLIA Kit (HUES01045)
Anti-NTF3 Antibody (CAB12476)	Human Neurotrophin-3 (NTF3) ELISA Kit
NTF3 Antibody (PACO10942)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)