Anti-NFKB1 Antibody (CAB11160)

Antibody Name:	Anti-NFKB1 Antibody
Antibody SKU:	CAB11160
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 740-964 of human NFKB1 (NP_001158884.1).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:200 IF 1:20 - 1:100 IP 1:20 - 1:50
Reactivity:	Human
Positive Samples:	Raji, MCF7

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 740-964 of human NFKB1 (NP_001158884.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AAGA DPLV ENFE PLYD LDDS WENA GEDE GVVP GTTP LDMA TSWQ VFDI LNGK PYEP EFTS DDLL AQGD MKQL AEDV KLQL YKLL EIPD PDKN WATL AQKL GLGI LNNA FRLS PAPS KTLM DNYE VSGG TVRE LVEA LRQM GYTE AIEV IQAA SSPV KTTS QAHS LPLS PAST RQQI DELR DSDS VCDS GVET SFRK LSFT ESLT SGAS LLTL NKMP HDYG QEGP L
Gene ID:	4790
Uniprot:	P19838
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	85kDa/105kDa
Observed MW:	120kDa

Synonyms:

NFKB1, CVID12, EBP-1, KBF1, NF-kB1, NF-kappa-B, NF-kappaB, NFKB-p105, NFKB-p50, NFkappaB, p105, p50

Background:

This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed.

UniProt Protein Function:	NFkB-p105: a transcription factor of the nuclear factor-kappaB ( NFkB) group. Undergoes cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of NFkB. NFkB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFkB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFkB has been associated with a number of inflammatory diseases while persistent inhibition of NFkB leads to inappropriate immune cell development or delayed cell growth. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Two alternatively spliced isoforms have been described.
UniProt Protein Details:	Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 4q24 Cellular Component: cytoplasm; cytosol; mitochondrion; neuron projection; nucleoplasm; nucleus Molecular Function:actinin binding; chromatin binding; heat shock protein binding; identical protein binding; protein binding; protein heterodimerization activity; protein homodimerization activity; transcription factor activity; transcription factor binding Biological Process: activation of NF-kappaB transcription factor; apoptosis; I-kappaB kinase/NF-kappaB cascade; inflammatory response; innate immune response; membrane protein intracellular domain proteolysis; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; negative regulation of apoptosis; negative regulation of cellular protein metabolic process; negative regulation of cholesterol transport; negative regulation of inflammatory response; negative regulation of interleukin-12 biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; nerve growth factor receptor signaling pathway; positive regulation of interferon type I production; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; response to copper ion; response to oxidative stress; stimulatory C-type lectin receptor signaling pathway; stress-activated MAPK cascade; T cell receptor signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway; transcription from RNA polymerase II promoter Disease: Immunodeficiency, Common Variable, 12
NCBI Summary:	This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed. [provided by RefSeq, Feb 2016]
UniProt Code:	P19838
NCBI GenInfo Identifier:	21542418
NCBI Gene ID:	4790
NCBI Accession:	P19838.2
UniProt Secondary Accession:	P19838,Q68D84, Q86V43, Q8N4X7, Q9NZC0, A8K5Y5, B3KVE8
UniProt Related Accession:	P19838
Molecular Weight:	85,520 Da
NCBI Full Name:	Nuclear factor NF-kappa-B p105 subunit
NCBI Synonym Full Names:	nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
NCBI Official Symbol:	NFKB1
NCBI Official Synonym Symbols:	p50; KBF1; p105; EBP-1; CVID12; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B
NCBI Protein Information:	nuclear factor NF-kappa-B p105 subunit
UniProt Protein Name:	Nuclear factor NF-kappa-B p105 subunit
UniProt Synonym Protein Names:	DNA-binding factor KBF1; EBP-1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
UniProt Gene Name:	NFKB1
UniProt Entry Name:	NFKB1_HUMAN

	Western blot analysis of extracts of various cell lines, using NFKB1 antibody (CAB11160) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human tonsil using NFKB1 antibody (CAB11160) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of MCF7 cells using NFKB1 antibody (CAB11160).
	Immunoprecipitation analysis of 150ug extracts of MCF-7 cells using 3ug NFKB1 antibody (CAB11160). Western blot was performed from the immunoprecipitate using NFKB1 antibody (CAB11160) at a dilition of 1:500.

Antibodies	ELISA Kits
Anti-NFKB1 Antibody (CAB14754)	Human NFKB1 / NFkB p105 / p50 ELISA Kit
Anti-NFKB1 Antibody (CAB6667)[KO Validated]	Human Nuclear factor NF-kappa-B p105 subunit (NFKB1) ELISA Kit
Anti-Phospho-NFKB1-S337 Antibody (CABP0125)	NF-kappaB p105/p50 Transcription Factor Activity Assay
Anti-Phospho-NFKB1-S927 Antibody (CABP0264)	NF-kappaB p105/p50 (Phospho-Ser927) Colorimetric Cell-Based ELISA Kit
Anti-Phospho-NFKB1-S893 Antibody (CABP0415)	NF-kappaB p105/p50 Colorimetric Cell-Based ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)