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Anti-NBN Antibody (CAB7703)

SKU:
CAB7703
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle
  • Cell Cycle Antibodies 2 Anti-NBN Antibody CAB7703
  • Cell Cycle Antibodies 2 Anti-NBN Antibody CAB7703
€139 - €419
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Description

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Antibody Name:Anti-NBN Antibody
Antibody SKU:CAB7703
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide of human NBN
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Jurkat, HeLa, MCF7, Mouse heart, Rat kidney
Immunogen:A synthetic peptide of human NBN
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:4683
Uniprot:O60934
Cellular Location:Chromosome, Nucleus, PML body, telomere
Calculated MW:84kDa
Observed MW:100kDa
Synonyms:NBN, AT-V1, AT-V2, ATV, NBS, NBS1, P95, nibrin
Background:Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation.
UniProt Protein Function:NBS1: a member of the MRE11/RAD50 double-strand break repair complex. Involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. Mutation results in the Nijmegen breakage syndrome (NBS), an autosomal recessive chromosomal instability syndrome.
UniProt Protein Details:

Protein type:Cell cycle regulation; DNA repair, damage

Chromosomal Location of Human Ortholog: 8q21

Cellular Component: cytosol; Mre11 complex; nuclear chromosome, telomeric region; nuclear inclusion body; nucleolus; nucleoplasm; nucleus; PML body; replication fork

Molecular Function:ATP-dependent DNA helicase activity; damaged DNA binding; protein binding; protein N-terminus binding; transcription factor binding

Biological Process: blastocyst growth; cell cycle arrest; cell proliferation; DNA damage checkpoint; DNA damage response, signal transduction by p53 class mediator; DNA duplex unwinding; DNA repair; double-strand break repair; double-strand break repair via homologous recombination; double-strand break repair via nonhomologous end joining; double-strand break repair via synthesis-dependent strand annealing; isotype switching; meiotic cell cycle; mitotic cell cycle checkpoint; mitotic cell cycle G2/M transition DNA damage checkpoint; neuromuscular process controlling balance; positive regulation of kinase activity; positive regulation of protein amino acid autophosphorylation; positive regulation of telomere maintenance; regulation of DNA replication initiation; telomere maintenance; telomeric 3' overhang formation

Disease: Aplastic Anemia; Leukemia, Acute Lymphoblastic; Nijmegen Breakage Syndrome

NCBI Summary:Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. [provided by RefSeq, Jul 2008]
UniProt Code:O60934
NCBI GenInfo Identifier:74762960
NCBI Gene ID:4683
NCBI Accession:O60934.1
UniProt Secondary Accession:O60934,O60672, Q32NF7, Q53FM6, Q63HR6, Q7LDM2, B2R626 B2RNC5,
UniProt Related Accession:O60934
Molecular Weight:84,959 Da
NCBI Full Name:Nibrin
NCBI Synonym Full Names:nibrin
NCBI Official Symbol:NBN  
NCBI Official Synonym Symbols:ATV; NBS; P95; NBS1; AT-V1; AT-V2  
NCBI Protein Information:nibrin
UniProt Protein Name:Nibrin
UniProt Synonym Protein Names:Cell cycle regulatory protein p95; Nijmegen breakage syndrome protein 1
Protein Family:Nibrin
UniProt Gene Name:NBN  
UniProt Entry Name:NBN_HUMAN
Anti-NBN Antibody (CAB7703)Western blot analysis of extracts of various cell lines, using NBN antibody (CAB7703) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 10s.
Anti-NBN Antibody (CAB7703)Immunofluorescence analysis of U2OS cells using NBN antibody (CAB7703) at dilution of 1:100. Blue: DAPI for nuclear staining.

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