Anti-NDUFA5 Antibody (CAB14751)

Antibody Name:	Anti-NDUFA5 Antibody [KO Validated]
Antibody SKU:	CAB14751
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-116 of human NDUFA5 (NP_004991.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-116 of human NDUFA5 (NP_004991.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAGV LKKT TGLV GLAV CNTP HERL RILY TKIL DVLE EIPK NAAY RKYT EQIT NEKL AMVK AEPD VKKL EDQL QGGQ LEEV ILQA EHEL NLAR KMRE WKLW EPLV EEPP ADQW KWPI
Gene ID:	4698
Uniprot:	Q16718
Cellular Location:	Matrix side, Mitochondrion inner membrane, Peripheral membrane protein
Calculated MW:	13kDa
Observed MW:	13kDa

Synonyms:

NDUFA5, B13, CI-13KD-B, CI-13kB, NUFM, UQOR13

Background:

This nuclear gene encodes a conserved protein that comprises the B13 subunit of complex I of the mitochondrial respiratory chain. The encoded protein localizes to the inner mitochondrial membrane, where it is thought to aid in the transfer of electrons from NADH to ubiquinone. Alternative splicing results in multiple transcript variants. There are numerous pseudogenes of this gene on chromosomes 1, 3, 6, 8, 9, 11, 12, and 16.

UniProt Protein Function:	NDUFA5: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I NDUFA5 subunit family.
UniProt Protein Details:	Protein type:EC 1.6.5.3; Mitochondrial; Energy Metabolism - oxidative phosphorylation; Oxidoreductase; EC 1.6.99.3 Chromosomal Location of Human Ortholog: 7q31.33 Cellular Component: mitochondrial inner membrane; mitochondrial respiratory chain complex I Molecular Function:NADH dehydrogenase (ubiquinone) activity Biological Process: cellular metabolic process; mitochondrial electron transport, NADH to ubiquinone
NCBI Summary:	This nuclear gene encodes a conserved protein that comprises the B13 subunit of complex I of the mitochondrial respiratory chain. The encoded protein localizes to the inner mitochondrial membrane, where it is thought to aid in the transfer of electrons from NADH to ubiquinone. Alternative splicing results in multiple transcript variants. There are numerous pseudogenes of this gene on chromosomes 1, 3, 6, 8, 9, 11, 12, and 16. [provided by RefSeq, Apr 2014]
UniProt Code:	Q16718
NCBI GenInfo Identifier:	2499316
NCBI Gene ID:	4698
NCBI Accession:	Q16718.3
UniProt Related Accession:	Q16718
Molecular Weight:
NCBI Full Name:	NADH dehydrogenase
NCBI Synonym Full Names:	NADH:ubiquinone oxidoreductase subunit A5
NCBI Official Symbol:	NDUFA5
NCBI Official Synonym Symbols:	B13; NUFM; UQOR13; CI-13kB; CI-13KD-B
NCBI Protein Information:	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5
UniProt Protein Name:	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5
UniProt Synonym Protein Names:	Complex I subunit B13; Complex I-13kD-B; CI-13kD-B; NADH-ubiquinone oxidoreductase 13 kDa-B subunit
UniProt Gene Name:	NDUFA5
UniProt Entry Name:	NDUA5_HUMAN

	Western blot analysis of extracts from normal (control) and NDUFA5 knockout (KO) HeLa cells, using NDUFA5 antibody (CAB14751) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
	Immunohistochemistry of paraffin-embedded human tonsil using NDUFA5 antibody (CAB14751) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using NDUFA5 antibody (CAB14751) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human gastric cancer using NDUFA5 antibody (CAB14751) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of NIH-3T3 cells using NDUFA5 Polyclonal Antibody (CAB14751) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

Anti-NDUFA5 Antibody (CAB3976)

NDUFA5 Antibody (PACO10778)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)