The NODAL Antibody (CAB9902) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in applications such as Western blotting.The Nodal Homolog protein is a member of the TGF-beta superfamily and is essential for diverse cellular processes, including stem cell maintenance and tissue patterning during development. By targeting the Nodal Homolog protein, researchers can gain insights into its functions and signaling pathways in various cell types, offering new avenues for studies in developmental biology and regenerative medicine.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
NODAL Antibody
SKU:
CAB9902
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
HTX5, NODAL
Positive Sample:
SH-SY5Y, HepG2, 293T, Rat liver
Cellular Localization:
Secreted.
Calculated MW:
40kDa
Observed MW:
44kDa
This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which regulates early embryonic development. This protein is required for maintenance of human embryonic stem cell pluripotency and may play a role in human placental development. Mutations in this gene are associated with heterotaxy, a condition characterized by random orientation of visceral organs with respect to the left-right axis.
Purification Method
Affinity purification
Gene ID
4838
RRID
AB_2770610
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using NODAL Rabbit pAb (CAB9902) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
