The NOLC1 Antibody (CAB5899) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high reactivity with human samples and is validated for use in Western blot applications. It specifically binds to the NOLC1 protein, allowing for precise detection and analysis in a variety of cell types.NOLC1 is a key player in cellular processes such as ribosome assembly and nucleolar organization, making it a crucial target for studies in molecular biology and cancer research.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NOLC1 Antibody
SKU:
CAB5899
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
P130, Srp40, NOPP130, NOPP140, NS5ATP13, NOLC1
Positive Sample:
A-549, HeLa, HepG2, MCF7
Cellular Localization:
Cytoplasm, Nucleus, Nucleolus.
Calculated MW:
74kDa
Observed MW:
120kDa
Enables protein heterodimerization activity and protein-macromolecule adaptor activity. Involved in neural crest cell development; neural crest formation; and regulation of translation. Located in fibrillar center.
Purification Method
Affinity purification
Gene ID
9221
RRID
AB_2766647
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using NOLC1 Rabbit pAb (CAB5899) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunofluorescence analysis of U-2 OS cells using NOLC1 Rabbit pAb (CAB5899) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
