The NOP58 Antibody (CAB4749) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced using rabbits, ensuring high reactivity and specificity for human samples. NOP58 is a key player in the biogenesis of ribosomes, the molecular machines responsible for protein synthesis in cells. It is essential for the maturation of ribosomal RNA and plays a crucial role in maintaining ribosome function. Dysregulation of NOP58 has been implicated in various diseases, including cancer and genetic disorders.This antibody is validated for use in Western blot applications, allowing for the detection and analysis of NOP58 in a variety of cell types.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
NOP58 Antibody
SKU:
CAB4749
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NOP5, HSPC120, NOP5/NOP58, NOP58
Positive Sample:
A375, NIH/3T3, K-562
Cellular Localization:
Nucleus, Nucleolus, Nucleoplasm.
Calculated MW:
60kDa
Observed MW:
65kDa
The protein encoded by this gene is a core component of box C/D small nucleolar ribonucleoproteins. Some box C/D small nucleolar RNAs (snoRNAs), such as U3, U8, and U14, are dependent upon the encoded protein for their synthesis. This protein is SUMOylated, which is necessary for high affinity binding to snoRNAs.
Purification Method
Affinity purification
Gene ID
51602
RRID
AB_2765846
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using NOP58 Rabbit pAb (CAB4749) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunofluorescence analysis of U2OS cells using NOP58 Rabbit pAb (CAB4749) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of H9C2 cells using NOP58 Rabbit pAb (CAB4749) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
