The NOX1 Antibody (CAB12309) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, boasts high reactivity with human samples and has been validated for use in Western blot applications.Nox1 is a key player in redox signaling and oxidative stress pathways, making it a subject of interest in studies related to oxidative damage, cell signaling, and inflammation. The Nox1 Polyclonal Antibody specifically targets the Nox1 protein, enabling precise detection and analysis in a variety of cell types.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
NOX1 Antibody
SKU:
CAB12309
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
This gene encodes a member of the NADPH oxidase family of enzymes responsible for the catalytic one-electron transfer of oxygen to generate superoxide or hydrogen peroxide. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Purification Method
Affinity purification
Gene ID
27035
RRID
AB_2759163
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HT-29 cells using NOX1 Rabbit pAb (CAB12309) at 1:3000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1 s.
Immunofluorescence analysis of HepG2 cells using NOX1 Rabbit pAb (CAB12309) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
