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Anti-NR5A2 Antibody (CAB13688)

SKU:
CAB13688
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
  • Epigenetics and Nuclear Signaling Antibodies 1 Anti-NR5A2 Antibody CAB13688
  • Epigenetics and Nuclear Signaling Antibodies 1 Anti-NR5A2 Antibody CAB13688
  • Epigenetics and Nuclear Signaling Antibodies 1 Anti-NR5A2 Antibody CAB13688
€139 - €419
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Description

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Antibody Name:Anti-NR5A2 Antibody
Antibody SKU:CAB13688
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human NR5A2 (NP_003813.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:BxPC-3, HepG2, A-549, Mouse brain, Mouse liver, Rat brain, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human NR5A2 (NP_003813.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSSN SDTG DLQE SLKH GLTP IVSQ FKMV NYSY DEDL EELC PVCG DKVS GYHY GLLT CESC KGFF KRTV QNNK RYTC IENQ NCQI DKTQ RKRC PYCR FQKC LSVG MKLE AVRA DRMR GGRN KFGP MYKR DRAL KQQK KALI
Gene ID:2494
Uniprot:O00482
Cellular Location:Nucleus
Calculated MW:42kDa/53kDa/56kDa/61kDa
Observed MW:61kDa
Synonyms:NR5A2, B1F, B1F2, CPF, FTF, FTZ-F1, FTZ-F1beta, LRH-1, LRH1, hB1F-2
Background:The protein encoded by this gene is a DNA-binding zinc finger transcription factor and is a member of the fushi tarazu factor-1 subfamily of orphan nuclear receptors. The encoded protein is involved in the expression of genes for hepatitis B virus and cholesterol biosynthesis, and may be an important regulator of embryonic development.
UniProt Protein Function:NR5A2: Binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development. Belongs to the nuclear hormone receptor family. NR5 subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:DNA-binding; Nuclear receptor; Transcription factor

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function:RNA polymerase II transcription factor activity, enhancer binding; protein binding; ligand-dependent nuclear receptor activity; DNA binding; zinc ion binding; sequence-specific DNA binding; phospholipid binding; steroid hormone receptor activity; transcription factor activity

Biological Process: transcription initiation from RNA polymerase II promoter; positive regulation of viral genome replication; intracellular receptor-mediated signaling pathway; bile acid metabolic process; positive regulation of transcription, DNA-dependent; endocrine pancreas development; regulation of cell proliferation; cholesterol homeostasis; embryonic development; homeostatic process; regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; gene expression

UniProt Code:O00482
NCBI GenInfo Identifier:7676156
NCBI Gene ID:2494
NCBI Accession:O00482.2
UniProt Secondary Accession:O00482,O95642, Q147U3, B4E2P3,
UniProt Related Accession:O00482
Molecular Weight:541
NCBI Full Name:Nuclear receptor subfamily 5 group A member 2
NCBI Synonym Full Names:nuclear receptor subfamily 5, group A, member 2
NCBI Official Symbol:NR5A2  
NCBI Official Synonym Symbols:B1F; CPF; FTF; B1F2; LRH1; LRH-1; FTZ-F1; hB1F-2; FTZ-F1beta  
NCBI Protein Information:nuclear receptor subfamily 5 group A member 2; nuclear receptor NR5A2; liver receptor homolog 1; liver receptor homolog-1; CYP7A promoter-binding factor; hepatocytic transcription factor hB1F-3; alpha-1-fetoprotein transcription factor; liver nuclear receptor homolog-1 variant 2; fetoprotein-alpha 1 (AFP) transcription factor; b1-binding factor, hepatocyte transcription factor which activates enhancer II of hepatitis B virus
UniProt Protein Name:Nuclear receptor subfamily 5 group A member 2
UniProt Synonym Protein Names:Alpha-1-fetoprotein transcription factor; B1-binding factor; hB1F; CYP7A promoter-binding factor; Hepatocytic transcription factor; Liver receptor homolog 1; LRH-1
Protein Family:Nuclear receptor subfamily
UniProt Gene Name:NR5A2  
UniProt Entry Name:NR5A2_HUMAN
Anti-NR5A2 Antibody (CAB13688)Western blot analysis of extracts of various cell lines, using NR5A2 antibody (CAB13688) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-NR5A2 Antibody (CAB13688)Immunohistochemistry of paraffin-embedded rat pancreas using NR5A2 Rabbit pAb (CAB13688) at dilution of 1:100 (40x lens).
Anti-NR5A2 Antibody (CAB13688)Immunohistochemistry of paraffin-embedded mouse pancreas using NR5A2 Rabbit pAb (CAB13688) at dilution of 1:100 (40x lens).

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