Anti-NSFL1C Antibody (CAB6677)

Antibody Name:	Anti-NSFL1C Antibody
Antibody SKU:	CAB6677
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-185 of human NSFL1C (NP_057227.2).

Application:	WB IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, U-251MG, Mouse kidney, Mouse brain, Rat brain, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-185 of human NSFL1C (NP_057227.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAE RQEA LREF VAVT GAEE DRAR FFLE SAGW DLQI ALAS FYED GGDE DIVT ISQA TPSS VSRG TAPS DNRV TSFR DLIH DQDE DEEE EEGQ RFYA GGSE RSGQ QIVG PPRK KSPN ELVD DLFK GAKE HGAV AVER VTKS PGET SKPR PFAG GGYR LGAA PEEE SAYV AGEK RQHS SQDV HVVL K
Gene ID:	55968
Uniprot:	Q9UNZ2
Cellular Location:	Chromosome, Golgi apparatus, Golgi stack, Nucleus
Calculated MW:	28kDa/37kDa/40kDa
Observed MW:	41kDa

Synonyms:

NSFL1C, P47, UBX1, UBXD10, UBXN2C, dJ776F14.1

Background:

N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 8.

UniProt Protein Function:	NSFL1C: Reduces the ATPase activity of VCP. Necessary for the fragmentation of Golgi stacks during mitosis and for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP- mediated formation of transitional endoplasmic reticulum (tER). Inhibits the activity of CTSL (in vitro). Belongs to the NSFL1C family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Vesicle Chromosomal Location of Human Ortholog: 20p13 Cellular Component: nucleoplasm; intermediate filament cytoskeleton; Golgi stack; cytoplasm; plasma membrane; chromosome; cytosol Molecular Function:ubiquitin binding; protein binding; protein phosphatase type 1 regulator activity; lipid binding Biological Process: proteasomal ubiquitin-dependent protein catabolic process; regulation of catalytic activity; nuclear envelope reassembly; Golgi organization and biogenesis; autophagic vacuole formation
NCBI Summary:	N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 8. [provided by RefSeq, May 2011]
UniProt Code:	Q9UNZ2
NCBI GenInfo Identifier:	41017512
NCBI Gene ID:	55968
NCBI Accession:	Q9UNZ2.2
UniProt Secondary Accession:	Q9UNZ2,Q5JXA4, Q5JXA5, Q7Z533, Q9H102, Q9NVL9, Q9UI06 A2A2L1, B2RD74,
UniProt Related Accession:	Q9UNZ2
Molecular Weight:	370
NCBI Full Name:	NSFL1 cofactor p47
NCBI Synonym Full Names:	NSFL1 (p97) cofactor (p47)
NCBI Official Symbol:	NSFL1C
NCBI Official Synonym Symbols:	P47; UBX1; UBXD10; UBXN2C; dJ776F14.1
NCBI Protein Information:	NSFL1 cofactor p47; SHP1 homolog; p97 cofactor p47; UBX domain-containing protein 2C
UniProt Protein Name:	NSFL1 cofactor p47
UniProt Synonym Protein Names:	UBX domain-containing protein 2C; p97 cofactor p47
Protein Family:	NSFL1 cofactor
UniProt Gene Name:	NSFL1C
UniProt Entry Name:	NSF1C_HUMAN

	Western blot analysis of extracts of various cell lines, using NSFL1C antibody (CAB6677) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of HeLa cells using NSFL1C antibody (CAB6677). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200ug extracts of A-549 cells, using 3 ug NSFL1C antibody (CAB6677). Western blot was performed from the immunoprecipitate using NSFL1C antibody (CAB6677) at a dilition of 1:1000.

ELISA Kits

HumanÂ NSFL1C (NSFL1Â cofactorÂ p47)Â ELISAÂ Kit (HUFI07476)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)