Anti-NCL Antibody (CAB5904)

Antibody Name:	Anti-NCL Antibody
Antibody SKU:	CAB5904
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 461-710 of human NCL (NP_005372.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	MCF7, A-549, THP-1

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 461-710 of human NCL (NP_005372.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LYYT GEKG QNQD YRGG KNST WSGE SKTL VLSN LSYS ATEE TLQE VFEK ATFI KVPQ NQNG KSKG YAFI EFAS FEDA KEAL NSCN KREI EGRA IRLE LQGP RGSP NARS QPSK TLFV KGLS EDTT EETL KESF DGSV RARI VTDR ETGS SKGF GFVD FNSE EDAK AAKE AMED GEID GNKV TLDW AKPK GEGG FGGR GGGR GGFG GRGG GRGG RGGF GGRG RGGF GGRG GFRG GRGG GGDH KPQG KKTK FE
Gene ID:	4691
Uniprot:	P19338
Cellular Location:	Cytoplasm, Nucleus, nucleolus
Calculated MW:	76kDa
Observed MW:	110kDa

Synonyms:	NCL, C23, Nsr1, nucleolin
Background:	Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.

UniProt Protein Function:	NCL: the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and preribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-RNA transcription and ribosome assembly.
UniProt Protein Details:	Protein type:Helicase; RNA-binding; Nucleolus; Nuclear receptor co-regulator; DNA-binding Chromosomal Location of Human Ortholog: 2q37.1 Cellular Component: cell cortex; membrane; nucleolus; nucleoplasm; nucleus; ribonucleoprotein complex Molecular Function:identical protein binding; protein binding; protein C-terminus binding; RNA binding; telomeric DNA binding Biological Process: angiogenesis
NCBI Summary:	Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008]
UniProt Code:	P19338
NCBI GenInfo Identifier:	90110781
NCBI Gene ID:	4691
NCBI Accession:	P19338.3
UniProt Secondary Accession:	P19338,Q53SK1, Q8NB06, Q9UCF0, Q9UDG1,
UniProt Related Accession:	P19338
Molecular Weight:	76,614 Da
NCBI Full Name:	Nucleolin
NCBI Synonym Full Names:	nucleolin
NCBI Official Symbol:	NCL
NCBI Official Synonym Symbols:	C23
NCBI Protein Information:	nucleolin
UniProt Protein Name:	Nucleolin
UniProt Synonym Protein Names:	Protein C23
Protein Family:	Nucleolin
UniProt Gene Name:	NCL
UniProt Entry Name:	NUCL_HUMAN

	Western blot analysis of extracts of various cell lines, using NCL antibody (CAB5904) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human liver cancer using NCL Rabbit pAb (CAB5904) at dilution of 1:100 (40x lens).
	Confocal immunofluorescence analysis of U2OS cells using NCL Polyclonal Antibody (CAB5904) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

NCL Antibody (PACO10760)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)