Anti-TIAL1 Antibody (CAB6075)

Antibody Name:	Anti-TIAL1 Antibody
Antibody SKU:	CAB6075
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 266-375 of human TIAL1 (NP_003243.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, HeLa, K-562

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 266-375 of human TIAL1 (NP_003243.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EGHV VKCY WGKE SPDM TKNF QQVD YSQW GQWS QVYG NPQQ YGQY MANG WQVP PYGV YGQP WNQQ GFGV DQSP SAAW MGGF GAQP PQGQ APPP VIPP PNQA GYGM ASYQ TQ
Gene ID:	7073
Uniprot:	Q01085
Cellular Location:	Cytoplasm, Cytoplasmic granule, Nucleus
Calculated MW:	41kDa/43kDa
Observed MW:	47kDa

Synonyms:

TIAL1, TCBP, TIAR

Background:

The protein encoded by this gene is a member of a family of RNA-binding proteins, has three RNA recognition motifs (RRMs), and binds adenine and uridine-rich elements in mRNA and pre-mRNAs of a wide range of genes. It regulates various activities including translational control, splicing and apoptosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The different isoforms have been show to function differently with respect to post-transcriptional silencing.

UniProt Protein Function:	TIAL1: a member of a family of RNA-binding proteins that possesses nucleolytic activity. A cytotoxic T cell granule-associated protein that has been shown to bind specifically to poly(A) homopolymers and to fragment DNA in permeabilized target cells. Members of this protein family may be involved in the induction of apoptosis. Two splice variant isoforms have been described.
UniProt Protein Details:	Protein type:RNA-binding; Apoptosis Chromosomal Location of Human Ortholog: 10q Cellular Component: stress granule; lysosome; cytoplasm; nucleus Molecular Function:RNA binding; nucleotide binding; AU-rich element binding Biological Process: regulation of transcription from RNA polymerase II promoter; apoptosis; stem cell division; positive regulation of cell proliferation; defense response; germ cell development
NCBI Summary:	The protein encoded by this gene is a member of a family of RNA-binding proteins, has three RNA recognition motifs (RRMs), and binds adenine and uridine-rich elements in mRNA and pre-mRNAs of a wide range of genes. It regulates various activities including translational control, splicing and apoptosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The different isoforms have been show to function differently with respect to post-transcriptional silencing. [provided by RefSeq, Jul 2008]
UniProt Code:	Q01085
NCBI GenInfo Identifier:	267131
NCBI Gene ID:	7073
NCBI Accession:	Q01085.1
UniProt Secondary Accession:	Q01085,P70318,
UniProt Related Accession:	Q01085
Molecular Weight:	375
NCBI Full Name:	Nucleolysin TIAR
NCBI Synonym Full Names:	TIA1 cytotoxic granule-associated RNA binding protein-like 1
NCBI Official Symbol:	TIAL1
NCBI Official Synonym Symbols:	TCBP; TIAR
NCBI Protein Information:	nucleolysin TIAR; T-cluster binding protein; TIA-1-related nucleolysin; aging-associated gene 7 protein
UniProt Protein Name:	Nucleolysin TIAR
UniProt Synonym Protein Names:	TIA-1-related protein
UniProt Gene Name:	TIAL1
UniProt Entry Name:	TIAR_HUMAN

	Western blot analysis of extracts of various cell lines, using TIAL1 antibody (CAB6075) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat lung using TIAL1 antibody (CAB6075) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human appendix using TIAL1 antibody (CAB6075) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spleen using TIAL1 antibody (CAB6075) at dilution of 1:100 (40x lens).

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)