Anti-NUDT2 Antibody (CAB6868)

Antibody Name:	Anti-NUDT2 Antibody
Antibody SKU:	CAB6868
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human NUDT2 (NP_001152.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	SKOV3, HL-60, Mouse kidney, Mouse heart, Mouse brain, Mouse liver, Rat kidney, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human NUDT2 (NP_001152.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MALR ACGL IIFR RCLI PKVD NNAI EFLL LQAS DGIH HWTP PKGH VEPG EDDL ETAL RETQ EEAG IEAG QLTI IEGF KREL NYVA RNKP KTVI YWLA EVKD YDVE IRLS HEHQ AYRW LGLE EACQ LAQF KEMK AALQ EGHQ FLCS IEA
Gene ID:	318
Uniprot:	P50583
Cellular Location:
Calculated MW:	16kDa
Observed MW:	17kDa

Synonyms:

NUDT2, APAH1

Background:

This gene encodes a member of the MutT family of nucleotide pyrophosphatases, a subset of the larger NUDIX hydrolase family. The gene product possesses a modification of the MutT sequence motif found in certain nucleotide pyrophosphatases. The enzyme asymmetrically hydrolyzes Ap4A to yield AMP and ATP and is responsible for maintaining the intracellular level of the dinucleotide Ap4A, the function of which has yet to be established. This gene may be a candidate tumor suppressor gene. Alternative splicing has been observed at this locus and four transcript variants, all encoding the same protein, have been identified.

UniProt Protein Function:	NUDT2: Asymmetrically hydrolyzes Ap4A to yield AMP and ATP. Plays a major role in maintaining homeostasis. Belongs to the Nudix hydrolase family.
UniProt Protein Details:	Protein type:Nucleotide Metabolism - pyrimidine; Apoptosis; EC 3.6.1.17; Hydrolase; Nucleotide Metabolism - purine Chromosomal Location of Human Ortholog: 9p13 Cellular Component: mitochondrial matrix Molecular Function:bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity; bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity; protein binding Biological Process: apoptosis; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; response to reactive oxygen species
NCBI Summary:	This gene encodes a member of the MutT family of nucleotide pyrophosphatases, a subset of the larger NUDIX hydrolase family. The gene product possesses a modification of the MutT sequence motif found in certain nucleotide pyrophosphatases. The enzyme asymmetrically hydrolyzes Ap4A to yield AMP and ATP and is responsible for maintaining the intracellular level of the dinucleotide Ap4A, the function of which has yet to be established. This gene may be a candidate tumor suppressor gene. Alternative splicing has been observed at this locus and four transcript variants, all encoding the same protein, have been identified. [provided by RefSeq, Sep 2011]
UniProt Code:	P50583
NCBI GenInfo Identifier:	1703326
NCBI Gene ID:	318
NCBI Accession:	P50583.3
UniProt Secondary Accession:	P50583,Q5T589, D3DRM0,
UniProt Related Accession:	P50583
Molecular Weight:	16,829 Da
NCBI Full Name:	Bis(5'-nucleosyl)-tetraphosphatase
NCBI Synonym Full Names:	nudix hydrolase 2
NCBI Official Symbol:	NUDT2
NCBI Official Synonym Symbols:	APAH1
NCBI Protein Information:	bis(5'-nucleosyl)-tetraphosphatase [asymmetrical]
UniProt Protein Name:	Bis(5'-nucleosyl)-tetraphosphatase [asymmetrical]
UniProt Synonym Protein Names:	Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase; Ap4A hydrolase; Ap4Aase; Diadenosine tetraphosphatase; Nucleoside diphosphate-linked moiety X motif 2; Nudix motif 2
Protein Family:	Nudix hydrolase
UniProt Gene Name:	NUDT2
UniProt Entry Name:	AP4A_HUMAN

	Western blot analysis of extracts of various cell lines, using NUDT2 antibody (CAB6868) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunofluorescence analysis of HeLa cells using NUDT2 antibody (CAB6868).

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)