Anti-OGDH Antibody (CAB6391)

Antibody Name:	Anti-OGDH Antibody
Antibody SKU:	CAB6391
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 148-427 of human OGDH (NP_001003941.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse kidney, Mouse heart, Mouse brain, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 148-427 of human OGDH (NP_001003941.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DPLG ILDA DLDS SVPA DIIS STDK LGFY GLDE SDLD KVFH LPTT TFIG GQES ALPL REII RRLE MAYC QHIG VEFM FIND LEQC QWIR QKFE TPGI MQFT NEEK RTLL ARLV RSTR FEEF LQRK WSSE KRFG LEGC EVLI PALK TIID KSSE NGVD YVIM GMPH RGRL NVLA NVIR KELE QIFC QFDS KLEA ADEG SGDV KYHL GMYH RRIN RVTD RNIT LSLV ANPS HLEA ADPV VMGK TKAE QFYC GDTE GKKV RPRE RRAR QIVK APCS SMEF RSPT
Gene ID:	4967
Uniprot:	Q02218
Cellular Location:	Mitochondrion matrix
Calculated MW:	48kDa/115kDa
Observed MW:	116kDa

Synonyms:

OGDH, AKGDH, E1k, OGDC

Background:

This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitochondrial matrix and uses thiamine pyrophosphate as a cofactor. A congenital deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

UniProt Protein Function:	OGDH: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3). Belongs to the alpha-ketoglutarate dehydrogenase family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Amino Acid Metabolism - tryptophan; Amino Acid Metabolism - lysine degradation; Oxidoreductase; Carbohydrate Metabolism - citrate (TCA) cycle; Mitochondrial; EC 1.2.4.2 Chromosomal Location of Human Ortholog: 7p14-p13 Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial membrane; cytosol; oxoglutarate dehydrogenase complex Molecular Function:chaperone binding; metal ion binding; heat shock protein binding; thiamin pyrophosphate binding; oxoglutarate dehydrogenase (succinyl-transferring) activity Biological Process: generation of precursor metabolites and energy; glycolysis; striatum development; succinyl-CoA metabolic process; tricarboxylic acid cycle; hippocampus development; pyramidal neuron development; thalamus development; 2-oxoglutarate metabolic process; cellular metabolic process; cerebellar cortex development; tangential migration from the subventricular zone to the olfactory bulb; NADH metabolic process; lysine catabolic process Disease: Alpha-ketoglutarate Dehydrogenase Deficiency
NCBI Summary:	This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitochondrial matrix and uses thiamine pyrophosphate as a cofactor. A congenital deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Sep 2009]
UniProt Code:	Q02218
NCBI GenInfo Identifier:	160332299
NCBI Gene ID:	4967
NCBI Accession:	Q02218.3
UniProt Secondary Accession:	Q02218,Q96DD3, Q9UDX0, B4E2U9, D3DVL0, E9PBM1,
UniProt Related Accession:	Q02218
Molecular Weight:	1023
NCBI Full Name:	2-oxoglutarate dehydrogenase, mitochondrial
NCBI Synonym Full Names:	oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
NCBI Official Symbol:	OGDH
NCBI Official Synonym Symbols:	E1k; OGDC; AKGDH
NCBI Protein Information:	2-oxoglutarate dehydrogenase, mitochondrial; OGDC-E1; oxoglutarate decarboxylase; 2-oxoglutarate dehydrogenase complex component E1; oxoglutarate dehydrogenase (succinyl-transferring)
UniProt Protein Name:	2-oxoglutarate dehydrogenase, mitochondrial
UniProt Synonym Protein Names:	2-oxoglutarate dehydrogenase complex component E1; OGDC-E1; Alpha-ketoglutarate dehydrogenase
UniProt Gene Name:	OGDH
UniProt Entry Name:	ODO1_HUMAN

	Western blot analysis of extracts of various cell lines, using OGDH antibody (CAB6391) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat ovary using OGDH antibody (CAB6391) at dilution of 1:100 (20x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using OGDH antibody (CAB6391) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using OGDH antibody (CAB6391). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-OGDH Antibody (CAB12477)	Human OGDH ELISA Kit
OGDH Antibody (PACO10995)	Human 2-oxoglutarate dehydrogenase, mitochondrial (OGDH) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)