Anti-OLR1 Antibody (CAB1639)

Antibody Name:	Anti-OLR1 Antibody
Antibody SKU:	CAB1639
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 58-273 of human OLR1 (NP_002534.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	OVCAR3, HeLa, HepG2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 58-273 of human OLR1 (NP_002534.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MQLS QVSD LLTQ EQAN LTHQ KKKL EGQI SARQ QAEE ASQE SENE LKEM IETL ARKL NEKS KEQM ELHH QNLN LQET LKRV ANCS APCP QDWI WHGE NCYL FSSG SFNW EKSQ EKCL SLDA KLLK INST ADLD FIQQ AISY SSFP FWMG LSRR NPSY PWLW EDGS PLMP HLFR VRGA VSQT YPSG TCAY IQRG AVYA ENCI LAAF SICQ KKAN LRAQ
Gene ID:	4973
Uniprot:	P78380
Cellular Location:	Cell membrane, Lipid-anchor, Membrane raft, Secreted, Single-pass type II membrane protein
Calculated MW:	20kDa/21kDa/30kDa
Observed MW:	26kDa

Synonyms:

OLR1, CLEC8A, LOX1, LOXIN, SCARE1, SLOX1

Background:

This gene encodes a low density lipoprotein receptor that belongs to the C-type lectin superfamily. This gene is regulated through the cyclic AMP signaling pathway. The encoded protein binds, internalizes and degrades oxidized low-density lipoprotein. This protein may be involved in the regulation of Fas-induced apoptosis. This protein may play a role as a scavenger receptor. Mutations of this gene have been associated with atherosclerosis, risk of myocardial infarction, and may modify the risk of Alzheimer's disease. Alternate splicing results in multiple transcript variants.

UniProt Protein Function:	OLR1: Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro- oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro- atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram- positive bacteria. Independent association genetic studies have implicated OLR1 gene variants in myocardial infarction susceptibility. OLR1 may be involved in Alzheimer disease (AD). Involvement in AD is however unclear: according to some authors (PubMed:12354387, PubMed:12810610 and PubMed:15976314), variations in OLR1 modify the risk of AD, while according to other (PubMed:15000751 and PubMed:15060104) they do not. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral; Receptor, misc. Chromosomal Location of Human Ortholog: 12p13.2-p12.3 Cellular Component: nucleoplasm; membrane; intracellular membrane-bound organelle; integral to plasma membrane; plasma membrane; receptor complex; lipid raft Molecular Function:low-density lipoprotein receptor activity; protein binding; carbohydrate binding Biological Process: cell death; receptor-mediated endocytosis; leukocyte adhesion; response to hydrogen peroxide; blood circulation; lipoprotein metabolic process; inflammatory response; proteolysis; blood coagulation; leukocyte migration Disease: Myocardial Infarction, Susceptibility To
NCBI Summary:	This gene encodes a low density lipoprotein receptor that belongs to the C-type lectin superfamily. This gene is regulated through the cyclic AMP signaling pathway. The encoded protein binds, internalizes and degrades oxidized low-density lipoprotein. This protein may be involved in the regulation of Fas-induced apoptosis. This protein may play a role as a scavenger receptor. Mutations of this gene have been associated with atherosclerosis, risk of myocardial infarction, and may modify the risk of Alzheimer's disease. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2010]
UniProt Code:	P78380
NCBI GenInfo Identifier:	73621335
NCBI Gene ID:	4973
NCBI Accession:	P78380.1
UniProt Secondary Accession:	P78380,Q2PP00, Q7Z484, A8K7V9, B4DI48, G3V1I4,
UniProt Related Accession:	P78380
Molecular Weight:	21,425 Da
NCBI Full Name:	Oxidized low-density lipoprotein receptor 1
NCBI Synonym Full Names:	oxidized low density lipoprotein (lectin-like) receptor 1
NCBI Official Symbol:	OLR1
NCBI Official Synonym Symbols:	LOX1; LOXIN; SLOX1; CLEC8A; SCARE1
NCBI Protein Information:	oxidized low-density lipoprotein receptor 1; hLOX-1; ox LDL receptor 1; lectin-type oxidized LDL receptor 1; scavenger receptor class E, member 1; C-type lectin domain family 8 member A; oxidized low-density lipoprotein receptor 1, soluble form
UniProt Protein Name:	Oxidized low-density lipoprotein receptor 1
UniProt Synonym Protein Names:	C-type lectin domain family 8 member A; Lectin-like oxidized LDL receptor 1; LOX-1; Lectin-like oxLDL receptor 1; hLOX-1; Lectin-type oxidized LDL receptor 1Cleaved into the following chain:Oxidized low-density lipoprotein receptor 1, soluble form
Protein Family:	Oxidized low-density lipoprotein receptor
UniProt Gene Name:	OLR1
UniProt Entry Name:	OLR1_HUMAN

	Western blot analysis of extracts of various cell lines, using OLR1 antibody (CAB1639) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded rat brain using OLR1 Antibody (CAB1639) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded human kidney using OLR1 Antibody (CAB1639) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse intestine using OLR1 Antibody (CAB1639) at dilution of 1:200 (40x lens).

Antibodies	ELISA Kits
Anti-OLR1 Antibody (CAB12060)	Human OxLDL / Oxidized low-density lipoprotein ELISA Kit
OLR1 Antibody (PACO11005)	Human LOX-1 (Lectin Like Oxidized Low Density Lipoprotein Receptor 1) CLIA Kit (HUES00926)
	Human Oxidized low-density lipoprotein receptor 1 (OLR1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)