ONPG Galactosidase Assay Kit (CV0012)

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SKU:
CV0012
€242

Description

ELISA Kit Technical ManualMSDS

ONPG Galactosidase Assay Kit

ONPG B-Galactosidase Assay Kit is an optimized, stable and convenient tool to quickly measure the levels of active Beta-galactosidase expressed in cells transfected with plasmids expressing Lac Z.

Lac Z is often used reporter gene in experiments transfection because the Beta-galactosidase is very resistant to proteolytic degradation and its activity is easily measured.

Beta-galactosidase performs the hydrolysis of orthonitrophenyl-beta-D-galactopyranoside (ONPG) to the ortho-nitrophenol (ONP). This ONP produces a bright yellow colour that is detected at an absorbance of 420 nm. The concentration of Beta-galactosidase is proportional to colour produced.

Kit Information:

Overview Description

Size:

500 assays

Features & Benefits:

  • Proven performance to quantify high expression level of beta-Gal.
  • Very stable: resistant to proteolytic degradation and easily assayed.
  • Convenient for all transfection assays.
  • Versatile: proven performance for cultured cells and tissues.
  • Fast and easy protocol.
  • Cost-effective.

Kit Contents:

  • ONGP Substrate solution
  • DTT
  • Buffer Lysis
  • Buffer Assay
  • Buffer Stop
  • Beta-galactosidase enzyme

Applications:

Measurement of Beta-Galactosidase activity in the lysates of transfected cells.

Shipping & Storage:

  • Shipped in: Gel Pack.
  • Storage: 4 ºC.
  • Shelf Life: 24 months from the date of purchase, if it is properly stored.

Safety:

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

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