Beta-Glucosidase Activity Assay Kit (BA0018)
beta-Glucosidase Activity Assay Kit - InformationAssay Genie's beta-Glucosidase Assay Kit is designed to measure beta-glucosidase activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl-beta-D-glucopyranoside that is hydrolyzed specifically by beta-glucosidase into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.
ApplicationsFor quantitative determination of beta-glucosidase activity and evaluation of drug effects on its metabolism.
beta-Glucosidase Activity Assay Kit - Key Features
- High sensitivity and wide linear range. Use 20 sample. The detection limit is 2 U/L, linear up to 250 U/L.
- Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of beta-glucosidase activity within 20 minutes.
- Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments.
beta-Glucosidase Activity Assay Kit - Data Sheet
|Kit Includes||Assay Buffer: 24 mL (pH 7.0) b-NPG Substrate: 1 mL Calibrator: 10 mL (equivalent to 250 U/L)|
|Kit Requires||Pipeting devices and accessories (e.g. multi-channel pipettor). Clear bottom 96-well plates and plate reader.|
|Method of Detection||OD405nm|
|Detection Limit||2 U/L|
|Protocol Length||20 min|
|Storage||Store all components at -20°C.|
|Shelf Life||6 months|
More Detailsbeta-GLUCOSIDASE is a glucosidase enzyme which acts upon 1->4 bonds linking two glucose or glucose-substituted molecules (i.e., the disaccharide cellobiose). beta-Glucosidases are required by organisms (some fungi, bacteria, termites) for consumption of cellulose. Lysozyme is also a beta-glucosidase and is present in tears to prevent bacterial infection of the eye. In humans, lower activity of a beta-glucosidase isoform (lysosomal gluco-cerebrosidase) has been related to Gaucher's disease and Parkinson's disease. Simple, direct and automation-ready procedures for measuring beta-glucosidase activity are becoming popular in Research and Drug Discovery.