Anti-P2RX2 Antibody (CAB7078)

Antibody Name:	Anti-P2RX2 Antibody
Antibody SKU:	CAB7078
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 60-320 of human P2RX2 (NP_733782.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, BT-474, HepG2, HL-60, Mouse liver, Mouse testis, Mouse heart, Rat liver, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 60-320 of human P2RX2 (NP_733782.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VFIV QKSY QESE TGPE SSII TKVK GITT SEHK VWDV EEYV KPPE GGSV FSII TRVE ATHS QTQG TCPE SIRV HNAT CLSD ADCV AGEL DMLG NGLR TGRC VPYY QGPS KTCE VFGW CPVE DGAS VSQF LGTM APNF TILI KNSI HYPK FHFS KGNI ADRT DGYL KRCT FHEA SDLY CPIF KLGF IVEK AGES FTEL AHKG GVIG VIIN WDCD LDLP ASEC NPKY SFRR LDPK HVPA SSGY NFRF AKYY KING TTTR TLIK A
Gene ID:	22953
Uniprot:	Q9UBL9
Cellular Location:	Cell membrane, Multi-pass membrane protein
Calculated MW:	41kDa/43kDa/44kDa/49kDa/51kDa/54kDa
Observed MW:	50-52kDa

Synonyms:	P2RX2, DFNA41, P2X2
Background:	The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel. Binding to ATP mediates synaptic transmission between neurons and from neurons to smooth muscle. Multiple transcript variants encoding distinct isoforms have been identified for this gene.

UniProt Protein Function:	P2X2: Binding of this ligand-gated ion channel to ATP mediates synaptic transmission between neurons and from neurons to smooth muscle. Belongs to the P2X receptor family. 7 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Channel, ligand-gated; Membrane protein, multi-pass; Membrane protein, integral; Channel, cation Chromosomal Location of Human Ortholog: 12q24.33 Cellular Component: presynaptic membrane; cell surface; cell soma; integral to plasma membrane; apical plasma membrane; postsynaptic density; dendritic spine; terminal button; integral to nuclear inner membrane; receptor complex Molecular Function:identical protein binding; zinc ion binding; mercury ion binding; drug binding; cadmium ion binding; ligand-gated ion channel activity; phosphoinositide binding; purinergic nucleotide receptor activity; nickel ion binding; copper ion binding; cobalt ion binding; ATP binding; ATP-gated cation channel activity Biological Process: urinary bladder smooth muscle contraction; peristalsis; sensory perception of taste; protein heterooligomerization; generation of action potential; skeletal muscle fiber development; positive regulation of calcium-mediated signaling; detection of hypoxic conditions in blood by carotid body chemoreceptors; response to carbohydrate stimulus; response to ATP; behavioral response to pain; sensory perception of sound; response to hypoxia; neuromuscular synaptic transmission; protein homooligomerization; neuromuscular junction development Disease: Deafness, Autosomal Dominant 41
NCBI Summary:	The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel. Binding to ATP mediates synaptic transmission between neurons and from neurons to smooth muscle. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Aug 2013]
UniProt Code:	Q9UBL9
NCBI GenInfo Identifier:	12643353
NCBI Gene ID:	22953
NCBI Accession:	Q9UBL9.1
UniProt Secondary Accession:	Q9UBL9,Q6V9R6, Q9NR37, Q9NR38, Q9UHD5, Q9UHD6, Q9UHD7 A6NGB4, A6NH93, A6NHC2, A6NHU3, A6NIG9,
UniProt Related Accession:	Q9UBL9
Molecular Weight:	471
NCBI Full Name:	P2X purinoceptor 2
NCBI Synonym Full Names:	purinergic receptor P2X, ligand-gated ion channel, 2
NCBI Official Symbol:	P2RX2
NCBI Official Synonym Symbols:	P2X2; DFNA41
NCBI Protein Information:	P2X purinoceptor 2; ATP receptor; P2X Receptor, subunit 2
UniProt Protein Name:	P2X purinoceptor 2
UniProt Synonym Protein Names:	ATP receptor; Purinergic receptor
Protein Family:	P2X purinoceptor
UniProt Gene Name:	P2RX2
UniProt Entry Name:	P2RX2_HUMAN

Western blot analysis of extracts of various cell lines, using P2RX2 antibody (CAB7078) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)