Anti-P2RX5 Antibody (CAB11710)

Antibody Name:	Anti-P2RX5 Antibody
Antibody SKU:	CAB11710
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 341-422 of human P2RX5 (NP_002552.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	NCI-H460, U-87MG, BxPC-3, K-562, Raji, Mouse heart, Mouse kidney, Mouse skeletal muscle, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 341-422 of human P2RX5 (NP_002552.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KKRE FYRD KKYE EVRG LEDS SQEA EDEA SGLG LSEQ LTSG PGLL GMPE QQEL QEPP EAKR GSSS QKGN GSVC PQLL EPHR ST
Gene ID:	5026
Uniprot:	Q93086
Cellular Location:	Membrane, Multi-pass membrane protein
Calculated MW:	44kDa/47kDa/48kDa
Observed MW:	115kDa

Synonyms:	P2RX5, LRH-1, P2X5, P2X5R
Background:	The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream gene, TAX1BP3 (Tax1 binding protein 3).

UniProt Protein Function:	P2X5: Receptor for ATP that acts as a ligand-gated ion channel. Belongs to the P2X receptor family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, multi-pass; Membrane protein, integral Chromosomal Location of Human Ortholog: 17p13.3 Cellular Component: cytoplasm; integral to nuclear inner membrane; plasma membrane Molecular Function:ATP-gated cation channel activity; ion channel activity; purinergic nucleotide receptor activity; transmembrane receptor activity Biological Process: blood coagulation; nervous system development; signal transduction; transport
NCBI Summary:	The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream gene, TAX1BP3 (Tax1 binding protein 3). [provided by RefSeq, Mar 2011]
UniProt Code:	Q93086
NCBI GenInfo Identifier:	209572778
NCBI Gene ID:	5026
NCBI Accession:	Q93086.4
UniProt Secondary Accession:	Q93086,O43450, O75540, Q308M5, Q59F38, Q8IXW4, Q93087 Q9NZV0, G5E981,
UniProt Related Accession:	Q93086
Molecular Weight:	44,531 Da
NCBI Full Name:	P2X purinoceptor 5
NCBI Synonym Full Names:	purinergic receptor P2X 5
NCBI Official Symbol:	P2RX5
NCBI Official Synonym Symbols:	P2X5; LRH-1; P2X5R
NCBI Protein Information:	P2X purinoceptor 5
UniProt Protein Name:	P2X purinoceptor 5
UniProt Synonym Protein Names:	ATP receptor; Purinergic receptor
Protein Family:	P2X purinoceptor
UniProt Gene Name:	P2RX5
UniProt Entry Name:	P2RX5_HUMAN

	Western blot analysis of extracts of various cell lines, using P2RX5 antibody (CAB11710) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunofluorescence analysis of Jurkat cells using P2RX5 Rabbit pAb (CAB11710) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of mouse spleen using P2RX5 Rabbit pAb (CAB11710) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

P2RX5 Antibody (PACO11038)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)