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Anti-P4HA2 Antibody (CAB4262)

SKU:
CAB4262
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
€139 - €419
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Description

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Antibody Name:Anti-P4HA2 Antibody
Antibody SKU:CAB4262
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 234-533 of human P4HA2 (NP_001136070.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:SKOV3, Mouse brain, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 234-533 of human P4HA2 (NP_001136070.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LSLD PSHE RAGG NLRY FEQL LEEE REKT LTNQ TEAE LATP EGIY ERPV DYLP ERDV YESL CRGE GVKL TPRR QKRL FCRY HHGN RAPQ LLIA PFKE EDEW DSPH IVRY YDVM SDEE IERI KEIA KPKL ARAT VRDP KTGV LTVA SYRV SKSS WLEE DDDP VVAR VNRR MQHI TGLT VKTA ELLQ VANY GVGG QYEP HFDF SRRP FDSG LKTE GNRL ATFL NYMS DVEA GGAT VFPD LGAA IWPK KGTA VFWY NLLR SGEG DYRT RHAA CPVL VGCK WVSN KWFH ERGQ EFLR PCGS TEVD
Gene ID:8974
Uniprot:O15460
Cellular Location:Endoplasmic reticulum lumen
Calculated MW:60kDa
Observed MW:65kDa
Synonyms:P4HA2, MYP25
Background:This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.
UniProt Protein Function:P4HA2: Catalyzes the post-translational formation of 4- hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins. Belongs to the P4HA family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Secreted; Amino Acid Metabolism - arginine and proline; Oxidoreductase; EC 1.14.11.2

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: intracellular membrane-bound organelle; endoplasmic reticulum lumen; endoplasmic reticulum; cytoplasm; nucleus

Molecular Function:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; procollagen-proline 4-dioxygenase activity; electron carrier activity; L-ascorbic acid binding; iron ion binding

Biological Process: peptidyl-proline hydroxylation to 4-hydroxy-L-proline

NCBI Summary:This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
UniProt Code:O15460
NCBI GenInfo Identifier:20455169
NCBI Gene ID:8974
NCBI Accession:O15460.1
UniProt Secondary Accession:O15460,Q8WWN0, D3DQ85, D3DQ86,
UniProt Related Accession:O15460
Molecular Weight:535
NCBI Full Name:Prolyl 4-hydroxylase subunit alpha-2
NCBI Synonym Full Names:prolyl 4-hydroxylase, alpha polypeptide II
NCBI Official Symbol:P4HA2  
NCBI Protein Information:prolyl 4-hydroxylase subunit alpha-2; 4-PH alpha 2; 4-PH alpha-2; C-P4Halpha(II); collagen prolyl 4-hydroxylase alpha(II); procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide II
UniProt Protein Name:Prolyl 4-hydroxylase subunit alpha-2
UniProt Synonym Protein Names:Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2
Protein Family:Prolyl 4-hydroxylase
UniProt Gene Name:P4HA2  
UniProt Entry Name:P4HA2_HUMAN
Anti-P4HA2 Antibody (CAB4262)Western blot analysis of extracts of various cell lines, using P4HA2 antibody (CAB4262) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

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