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Pan-AKT Rabbit Polyclonal Antibody (CAB18120)

SKU:
CAB18120
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Pan-AKT Rabbit Polyclonal Antibody
SKU:CAB18120
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 311-480 of human AKT1/AKT2/AKT3 (NP_005154.2).
Sequence:GTPE YLAP EVLE DNDY GRAV DWWG LGVV MYEM MCGR LPFY NQDH EKLF ELIL MEEI RFPR TLGP EAKS LLSG LLKK DPKQ RLGG GSED AKEI MQHR FFAG IVWQ HVYE KKLS PPFK PQVT SETD TRYF DEEF TAQM ITIT PPDQ DDSM ECVD SERR PHFP QFSY SASG TA
Tested Applications:WB IHC-P IF/ICC IP ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:100 IP,0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
Synonyms:AKT1/AKT2/AKT3; Pan-Akt
Positive Sample:MCF7,Mouse brain,Rat brain
Conjugate:Unconjugated
Cellular Localization:ciliary basal body, cytoplasm, cytosol, microtubule cytoskeleton, mitochondrion, nucleoplasm, nucleus, plasma membrane, spindle
Calculated MW:48kDa/55kDa/51kDa/54kDa
Observed MW:60kDa

Human AKT serine-threonine protein kinase family includes three members AKT1,AKT2, AKT3, which are also often referred to as protein kinase B alpha, beta, and gamma. These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine/threonine-specific kinase domain and a C-terminal regulatory domain. These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K). AKT/PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. AKT proteins are recruited to the cell membrane by phosphatidylinositol 3,4,5-trisphosphate (PIP3) after phosphorylation of phosphatidylinositol 4,5-bisphosphate (PIP2) by PI3K. Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene.

Purification Method:Affinity purification
Gene ID:207/208/10000
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Pan-Akt Rabbit pAb (CAB18120) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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